Cell surface antigens of human renal cancer defined by mouse monoclonal antibodies: identification of tissue-specific kidney glycoproteins.
Cell surface antigens of human renal cancer defined by mouse monoclonal antibodies: identification of tissue-specific kidney glycoproteins.
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由小鼠单克隆抗体定义的人肾癌细胞表面抗原：组织特异性肾糖蛋白的鉴定。
DOI：
10.1073/pnas.78.8.5122
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发表时间：
1981
影响因子：
11.1
通讯作者：
Old,LJ
中科院分区：
文献类型：
--
作者：
Ueda,R;Ogata,S;Morrissey,DM;Finstad,CL;Szkudlarek,J;Whitmore,WF;Oettgen,HF;Lloyd,KO;Old,LJ
17个单克隆抗体来自与脾细胞的免疫小鼠与肾癌的建立培养系的融合，确定了9个细胞表面抗原系统。其中6个系统（gp 160、S25、gp 120 r、gp 120 nr、gp 115和V1）代表以前未描述的抗原。其他三个系统与HLA-A、-B和-C重链以及A和B血型抗原有关。最受限制的新描述的抗原是gp 160，S25和gp 120 r。这些决定簇仅在正常和恶性的肾源性细胞上发现，并且代表人肾的分化抗原。除了这些抗原中的两种的分子量的差异之外，gp 160、S25和gp 120 r可以基于在一组培养的肾癌和正常肾上皮和胎儿肾细胞上的差异表达来区分。携带gp 120 r的糖蛋白与肾脏gp 120 nr共享一个决定簇（如检测gp 120 r和gp 120 nr的单克隆抗体的连续沉淀所示），但gp 120 nr存在于更广泛的细胞类型中，包括成纤维细胞和来自肺癌、膀胱癌和结肠癌的细胞系。另外两个新系统，gp 115和V1，具有广泛存在的分化抗原的特征，但可以通过分子量、热稳定性（V1是热稳定决定子）和不同来源的细胞类型上的差异表达来彼此区分，并与gp 120 nr区分。
Seventeen monoclonal antibodies derived from fusions with spleen cells of mice immunized with established culture lines of renal cancers identified nine cell-surface antigenic systems. Six of the systems (gp160, S25, gp120r, gp120nr, gp115, and V1) represent antigens not previously described. The other three systems are related to HLA-A, -B, and -C heavy chain and A and B blood group antigens. The most restricted of the newly described antigens are gp160, S25, and gp120r. These determinants are found only on cells of renal origin, both normal and malignant, and represent differentiation antigens of human kidney. In addition to the difference in the molecular weight of two of these antigens, gp160, S25, and gp120r can be distinguished on the basis of differential expression on a panel of cultured renal cancers and normal kidney epithelium and fetal kidney cells. Glycoproteins bearing gp120r share a determinant with renal gp120nr (as indicated by sequential precipitations with monoclonal antibodies that detect gp120r and gp120nr), but gp120nr is found on a broader range of cell types, including fibroblasts and cell lines derived from lung, bladder, and colon cancers. The two other new systems, gp115 and V1, have characteristics of broadly occurring differentiation antigens but can be distinguished from each other and from gp120nr by differences in molecular weight, heat stability (V1 is a heat-stable determinant), and differential expression on cell types of diverse origin.