Physiological and molecular features of the pathosystem Arabidopsis thaliana L.-Sclerotinia sclerotiorum Libert
Physiological and molecular features of the pathosystem Arabidopsis thaliana L.-Sclerotinia sclerotiorum Libert
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DOI：
10.1111/j.1744-7909.2006.00181.x-i1
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发表时间：
2006-01-01
影响因子：
11.4
通讯作者：
He, ZH
中科院分区：
文献类型：
--
作者：
Dai, FM;Xu, T;He, ZH
核盘菌（Sclerotinia sclerotiorum Libert）是世界范围内许多农作物的主要病害，由于缺乏抗病品种，造成了巨大的经济损失。对S.核盘菌和植物对病原体反应的分子基础知之甚少。本研究以S.拟南芥中的菌核病感染，一种对这种真菌高度敏感的植物进行了分析。此外，还研究了宿主的防御激活。研究了一种简便的谷子接种法。sclerotiorum在拟南芥中的表达。真菌迅速感染植物，可能是通过球或漏斗状感染结构。可见的症状在24小时内发生，接种后72小时植物被杀死。纤维素酶是引起寄主组织腐烂的主要酶，它是由S. sclerotiorum以pH依赖的方式。草酸是该菌分泌的另一种致病因子，可引起叶片坏死。链球菌感染在拟南芥中，强烈诱导了病程相关（PR）蛋白β-1，3-葡聚糖酶和几丁质酶的产生。此外，PR基因PDF.1被诱导，而不是PR 1，表明病原菌激活茉莉酸/乙烯依赖的基础防御，这与其坏死营养特性一致。该系统为紫茎泽兰的致病系统。scierotiorum为分析S. scierotiorum和其他作物，从而促进控制这种病原体的遗传操作技术。
The fungal pathogen Sclerotinia sclerotiorum Libert causes rot diseases on many crops worldwide and large economic losses occur frequently because of a lack of resistant varieties. The pathogenesis of S. sclerotiorum and the molecular basis of plant responses to the pathogen are poorly understood. In the present investigation, the process of S. sclerotiorum infection in Arabidopsis thaliana L., a plant that is highly susceptible to this fungus, was analysed. In addition, the defense activation in the host was investigated. A convenient inoculation method using millet grain was developed for S. sclerotiorum in Arabidopsis. The fungus rapidly infected the plants, probably through ball- or cushion-like infection structures. Visible symptoms developed within 24 h and plants were killed 72 h after inoculation. Cellulase, the main enzyme that caused host tissues to rot, was secreted by S. sclerotiorum in a pH-dependent manner. Oxalic acid, another pathogenic factor secreted by the fungus, induced necrotic lesions on the leaves. Infection with S. sclerotiorum, strongly induced the production of the pathogenesis-related (PR) proteins beta-1,3-glucanase and chitinase in Arabidopsis. Furthermore, the PR gene PDF.1 was induced, but not PR1, indicating that the pathogen activated basal defense of jasmonic acid/ethylene dependence, which is consistent with its necrotrophic characteristics. This pathosystem for Arabidopsis-S. scierotiorum could provide an approach for the analysis of the interactions between S. scierotiorum and other crops, thereby facilitating genetic manipulation techniques for controlling this pathogen.