Detection of antibodies to Epstein-Barr virus antigens by enzyme-linked immunosorbent assay.
Detection of antibodies to Epstein-Barr virus antigens by enzyme-linked immunosorbent assay.
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通过酶联免疫吸附测定检测 Epstein-Barr 病毒抗原的抗体。
DOI：
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发表时间：
1982
影响因子：
6.4
通讯作者：
H. Rabin
中科院分区：
文献类型：
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作者：
R. Hopkins;T. Witmer;R. Neubauer;H. Rabin
描述了用于EB病毒（EBV）相关核抗原（EBNA）、病毒衣壳抗原（VCA）和早期抗原（EA）抗体的血清学分析的酶联免疫吸附试验（ELISA）。通过使用充分表征的人血清证明了每种ELISA的特异性，所述人血清通过免疫荧光试验显示对所研究的三种病毒抗原中的一种或多种的抗体具有不同的反应性。EBNA的ELISA是4至256倍的灵敏度比免疫荧光检测与所有33 EBNA阳性血清测试。VCA和EA的ELISA也比免疫荧光法更敏感：大约50%的血清检测显示较高的抗体滴度。通过免疫荧光测定对所有三种抗原呈阴性的血清通过ELISA对每种抗原也呈阴性。这些EB病毒的ELISA快速，敏感，客观，从而提供了新的和有价值的方法检测抗体的EB病毒相关抗原。
An enzyme-linked immunosorbent assay (ELISA) is described for the serologic analysis of antibodies to Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA), viral capsid antigen (VCA), and early antigen (EA). The specificity of each of the ELISAs was demonstrated by the use of well-characterized human sera shown by immunofluorescence assay to be variously reactive for antibodies to one or more of the three viral antigens studied. The ELISA for EBNA was four to 256 times more sensitive than immunofluorescence assays with all 33 EBNA-positive sera tested. The ELISAs for VCA and EA were also more sensitive than immunofluorescence assays: approximately 50% of the sera tested showed higher antibody titers. Sera that were negative for all three antigens by immunofluorescence assay were also negative by ELISA for each antigen. These ELISAs for EBV are rapid, sensitive, and objective and thus provide new and valuable methods for the detection of antibodies to EBV-related antigens.