Does reproduction protect against oxidative stress?
Does reproduction protect against oxidative stress?
复制标题
DOI：
10.1242/jeb.114116
复制
发表时间：
2014-12-01
影响因子：
2.8
通讯作者：
Eens, Marcel
中科院分区：
文献类型：
--
作者：
Costantini, David;Casasole, Giulia;Eens, Marcel
生活史理论的一个核心原则是，父母在生殖投资与身体维护投资之间进行了权衡。氧化应激被认为是这种权衡的重要调节剂。然而，对这一假设的实验支持被证明是矛盾的。在这项研究中，我们操纵的雏鸟饲养努力的圈养金丝雀（金丝雀）孵化后不久，他们的雏鸟使用育雏大小的操作，以测试是否增加雏鸟饲养努力转化为增加氧化损伤，增加血浆铜蓝蛋白（这是上调氧化损伤）和减少巯基抗氧化剂。我们还比较了繁殖鸟类与非繁殖鸟类的血液氧化应激水平，这是大多数研究总是未能包括在繁殖的氧化成本测试中的一个关键方面。与非繁殖金丝雀和预处理值相比，血浆氧化损伤（活性氧代谢产物和蛋白质羰基）在繁殖金丝雀中降低，无论性别和育雏大小。相比之下，在实验中，非繁殖鸟类的氧化损伤没有改变。在两个处理组中，血浆中的铜蓝蛋白活性以及红细胞中的非蛋白和蛋白硫醇在整个实验期间均未发生变化。我们的研究结果表明，生殖可能会导致血液氧化应激减少，而不是增加。我们的研究结果可以解释一些不一致的，到目前为止，在实验测试的氧化成本繁殖假说。
A central principle of life-history theory is that parents trade investment in reproduction against that in body maintenance. One physiological cost thought to be important as a modulator of such trade-off is oxidative stress. Experimental support for this hypothesis has, however, proved to be contradictory. In this study, we manipulated the nestling rearing effort of captive canaries (Serinus canaria) soon after the hatching of their nestlings using a brood-size manipulation to test whether an increase in nestling rearing effort translates into an increase in oxidative damage, an increase in ceruloplasmin (which is upregulated in response to oxidative damage) and a decrease in thiol antioxidants. We also compared the blood oxidative stress level of reproducing birds with that of non-reproducing birds, a crucial aspect that most studies have invariably failed to include in tests of the oxidative cost of reproduction. Compared with non-breeding canaries and pre-manipulation values, plasma oxidative damage (reactive oxygen metabolites and protein carbonyls) decreased in breeding canaries irrespective of sex and brood size. In contrast, oxidative damage did not change in non-breeding birds over the experiment. Ceruloplasmin activity in plasma and both non-protein and protein thiols in red blood cells did not change throughout the experiment in both treatment groups. Our results suggest that reproduction may result in decreased rather than increased blood oxidative stress. Our results may explain some of the inconsistencies that have so far been reported in experimental tests of the oxidative cost of reproduction hypothesis.