SELECTIVE GENOTYPING FOR DETERMINATION OF LINKAGE BETWEEN A MARKER LOCUS AND A QUANTITATIVE TRAIT LOCUS
SELECTIVE GENOTYPING FOR DETERMINATION OF LINKAGE BETWEEN A MARKER LOCUS AND A QUANTITATIVE TRAIT LOCUS
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DOI：
10.1007/bf00222881
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发表时间：
1992-11-01
影响因子：
5.4
通讯作者：
SOLLER, M
中科院分区：
文献类型：
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作者：
DARVASI, A;SOLLER, M
“选择性基因分型”是当通过仅对来自整个样品群体的高和低表型尾部的个体进行基因分型来确定影响某些特定性状的标记基因座和数量性状基因座（QTL）之间的连锁时使用的术语。选择性基因分型可以显著减少给定功效的基因分型个体的数量，代价是增加表型分型个体的数量。从使给定实验能力的成本最小化的观点来看，基因分型个体的最佳比例强烈地取决于相对于个体的饲养和性状评价成本，对实验中包括的所有标记物对个体进行完全基因分型的成本（包括获得DNA样品的成本）。然而，在单性状研究中，对超过25%的群体进行基因分型几乎是没有用的。结果表明，所观察到的数量性状值的差异与替代标记基因型在选定的人口可以远远大于实际的基因效应在数量性状位点时，整个人口被认为是。提供了将选择性基因分型下观察到的差异转换为实际基因效应的表达式和图。
"Selective genotyping" is the term used when the determination of linkage between marker loci and quantitative trait loci (QTL) affecting some particular trait is carried out by genotyping only individuals from the high and low phenotypic tails of the entire sample population. Selective genotyping can markedly decrease the number of individuals genotyped for a given power at the expense of an increase in the number of individuals phenotyped. The optimum proportion of individuals genotyped from the point of view of minimizing costs for a given experimental power depends strongly on the cost of completely genotyping an individual for all of the markers included in the experiment (including the costs of obtaining a DNA sample) relative to the cost of rearing and trait evaluation of an individual. However, in single trait studies, it will almost never be useful to genotype more than the upper and lower 25% of a population. It is shown that the observed difference in quantitative trait values associated with alternative marker genotypes in the selected population can be much greater than the actual gene effect at the quantitative trait locus when the entire population is considered. An expression and a figure is provided for converting observed differences under selective genotyping to actual gene effects.