Controlled self assembly of collagen nanoparticle
Controlled self assembly of collagen nanoparticle
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DOI：
10.1007/s11051-011-0327-x
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发表时间：
2011-03
影响因子：
2.5
通讯作者：
M. Papi;V. Palmieri;G. Maulucci;G. Arcòvito;E. Greco;G. Quintiliani;M. Fraziano;M. Spirito
中科院分区：
文献类型：
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作者：
M. Papi;V. Palmieri;G. Maulucci;G. Arcòvito;E. Greco;G. Quintiliani;M. Fraziano;M. Spirito
近年来，载体介导的药物递送已经成为治疗各种病理的有力方法。传统药物和新型药物的治疗指数通过由于药物靶向特定组织、细胞或细胞内区室而增加特异性、控制释放动力学、保护活性剂或以上的组合而增强。胶原蛋白具有生物相容性、低毒性、可生物降解性和弱抗原性等特点，是一种重要的医用生物材料，是理想的蛋白质药物载体。虽然已经进行了许多尝试，但需要进一步的工作来生产具有所需尺寸的完全生物相容性的胶原水凝胶，并且能够在特定靶点上释放药物。在这篇文章中，我们提出了一种新的方法来获得由DMPC脂质体包围的聚合胶原制成的球形颗粒。脂质体允许在胶原聚合期间控制颗粒尺寸和胶凝环境。此外，基于光学的方法来可视化和量化所提出的协议的每一个步骤进行了详细的讨论。
In recent years carrier-mediated drug delivery has emerged as a powerful methodology for the treatment of various pathologies. The therapeutic index of traditional and novel drugs is enhanced via the increase of specificity due to targeting of drugs to a particular tissue, cell or intracellular compartment, the control over release kinetics, the protection of the active agent, or a combination of the above. Collagen is an important biomaterial in medical applications and ideal as protein-based drug delivery platform due to its special characteristics, such as biocompatibility, low toxicity, biodegradability, and weak antigenicity. While some many attempts have been made, further work is needed to produce fully biocompatible collagen hydrogels of desired size and able to release drugs on a specific target. In this article we propose a novel method to obtain spherical particles made of polymerized collagen surrounded by DMPC liposomes. The liposomes allow to control both the particles dimension and the gelling environment during the collagen polymerization. Furthermore, an optical based method to visualize and quantify each step of the proposed protocol is detailed and discussed.