Phytochemical Composition, Antioxidant and Antitumor Activities of some Date Palm Pollen Extracts
Phytochemical Composition, Antioxidant and Antitumor Activities of some Date Palm Pollen Extracts
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DOI：
10.21608/ejchem.2022.144841.6320
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发表时间：
2023-05-01
影响因子：
1.3
通讯作者：
Mhsen, Mona Mohamed Abdel
中科院分区：
文献类型：
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作者：
Abdallah, Walid Elsayed;Awad, Hanem M.;Mhsen, Mona Mohamed Abdel
本研究的目的是研究采自埃及沙基亚市阿布哈马德的椰枣花粉颗粒(DPP)的植物化学成分和抗氧化能力。用气相色谱对凤凰属植物花粉中的脂类成分进行了分析，鉴定出以棕榈酸为主要成分的9种脂肪酸和以十五烷为主要成分的含有一系列碳氢化合物、甾醇的不皂化部分。甲醇提取物(ME)的总酚含量(TPC)和总黄酮含量(TFC)分别用Folin-Ciocalteau试剂法和三氯化铝法进行测定。采用高效液相色谱法对23种酚类化合物和20种黄酮类化合物进行了鉴定和定量，为其生物活性研究提供科学依据。结果表明，该乙醇提取物的总酚含量为62.1&PLUSMN；GAE为1.8 mg/g，总黄酮含量为90.2&PLUSMN；2.3 mg QE/g。以阿霉素为参比药物，DPP颗粒的乙醇提取物和乙酸乙酯提取物在浓度为25mU；M时对人RPE-1和MCF-7细胞显示出较强的抗氧化活性和较高的细胞毒活性。
The aim of the present study was to investigate the phytochemical composition and the antioxidant potential of the Date Palm pollen grains (DPP) collected from Abu-Hamad, Sharkia, Egypt. Investigation of the lipid constituents of pollen grains of Phoenix dactylifera by GLC analyses led to identification of the fatty acids mixture which consists of nine fatty acids with palmitic acid as a major and the unsaponifiable fraction that contains a series of hydrocarbons, sterols, with pentadecane as a major. Estimation of total phenolic content (TPC) and total flavonoid content (TFC) of the methanolic extract (ME) were performed by Folin-Ciocalteau reagent and aluminium chloride methods respectively. Twenty three phenolic compounds and twenty flavonoid compounds were identified and quantified by HPLC to provide the scientific basis for its biological activity. The results revealed that the methanolic extract had total phenolic content value of 62.1 & PLUSMN; 1.8 mg GAE/g and total flavonoid content value of 90.2 & PLUSMN; 2.3mg QE/g. Antioxidant activity was screened by DPPH assay. The methanolic and ethyl acetate extracts of the DPP grains showed potent antioxidant activity and high cytotoxic activities at a concentration of 25 & mu;M against RPE-1 and MCF-7 human cell lines using MTT assay, compared to doxorubicin as a reference drug.