Biological imaging of chemical bonds by stimulated Raman scattering microscopy
Biological imaging of chemical bonds by stimulated Raman scattering microscopy
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DOI：
10.1038/s41592-019-0538-0
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发表时间：
2019-09-01
期刊：
影响因子：
48
通讯作者：
Min, Wei
中科院分区：
文献类型：
--
作者：
Hu, Fanghao;Shi, Lixue;Min, Wei
所有的分子都由化学键组成，从绘制这些键的时空动力学可以学到很多东西。自十年前发明以来，受激拉曼散射（SRS）显微镜已成为一种强大的模式，用于成像化学键，具有高灵敏度，分辨率，速度和特异性。我们介绍了SRS显微镜的基本原理，并回顾了SRS显微镜和成像探针的创新。我们强调了令人兴奋的生物应用的例子，并分享了我们对这项技术未来潜在突破的愿景。
All molecules consist of chemical bonds, and much can be learned from mapping the spatiotemporal dynamics of these bonds. Since its invention a decade ago, stimulated Raman scattering (SRS) microscopy has become a powerful modality for imaging chemical bonds with high sensitivity, resolution, speed and specificity. We introduce the fundamentals of SRS microscopy and review innovations in SRS microscopes and imaging probes. We highlight examples of exciting biological applications, and share our vision for potential future breakthroughs for this technology.