Time-course of programmed cell death during leaf senescence in Eucommia ulmoides
Time-course of programmed cell death during leaf senescence in Eucommia ulmoides
复制标题
杜仲叶片衰老过程中细胞程序性死亡的时程
DOI：
10.1007/s10265-002-0063-5
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发表时间：
2003-02-01
影响因子：
2.8
通讯作者：
Cui, KM
中科院分区：
文献类型：
--
作者：
Cao, J;Jiang, F;Cui, KM
杜仲叶：橄榄。在4月至11月期间收获，检查生长和衰老期间的程序性细胞死亡（PCD）。叶子在4月发育，在5月下旬完全展开，直到11月才开始开裂。落叶还保留着绿色。结果表明：(1)叶肉细胞在9月至11月间细胞核逐渐减少，(2)8月间细胞核出现TUNEL阳性信号，(3)9月和10月间出现阶梯状DNA断裂，(4)在这几个月中出现了20 kda的Ca（2+）依赖性DNA酶。落叶中叶肉细胞核未见完整，维管束周围有少量细胞有核。因此：(1)杜仲叶细胞发生程序性细胞死亡（PCD），(2)叶肉细胞PCD的进程持续2个月以上，(3)自然衰老叶细胞PCD是不同步的。
Leaves of Eucommia ulmoidesOliv. harvested between April to November were examined for programmed cell death (PCD) during growth and senescence. Leaves developed in April, becoming fully expanded in late May, remaining unchanged until November when they started to dehisce. Falling leaves retained a green color. Our results showed that (1) mesophyll cells gradually reduced their nuclei from September to November, (2) positive TUNEL signals appeared on the nuclei from August, (3) ladder-like DNA fragmentation occurred in September and October, and (4) a 20-kDa Ca(2+)-dependent DNase appeared in these same months. In fallen leaves, intact mesophyll cell nuclei could not be detected, but a few cells around the vascular bundle had nuclei. Therefore, (1) programmed cell death (PCD) of leaf cells occurred in the leaves of E. ulmoides, (2) the progress of mesophyll cell PCD lasted for more than 2 months, and (3) PCD of leaf cells was asynchronous in natural senescing leaves.