Cryopreserved mouse hepatocytes retain regenerative capacity in vivo.
Cryopreserved mouse hepatocytes retain regenerative capacity in vivo.
复制标题
冷冻保存的小鼠肝细胞保留体内再生能力。
DOI：
10.1016/s0016-5085(00)70221-6
复制
发表时间：
2000
期刊：
影响因子：
29.4
通讯作者：
Sandgren,EP
中科院分区：
文献类型：
--
作者：
Jamal,HZ;Weglarz,TC;Sandgren,EP
Background & AimsSubstitution of hepatocyte transplantation for whole liver transplants in selected individuals with liver disease could significantly expand the number of patients to benefit from use of scarce donor livers. However, successful hepatocyte transplantation may require that donor cells retain normal functional and proliferative capabilities and that they be readily available. Banking of cryopreserved hepatocytes would fulfill the latter requirement. Cryopreservation protocols have been developed that minimize hepatocyte injury and allow preservation of metabolic activity. The aim of this study was to assess cryopreserved hepatocyte proliferative capacity in vivo after thawing.MethodsFresh and frozen/thawed mouse hepatocytes were transferred separately into the livers of recipient mice with transgene-induced liver disease, an environment that is permissive for clonal expansion of donor cell populations. Fresh and cryopreserved donor cells were compared for their ability to proliferate and replace damaged parenchyma.ResultsAlthough cryopreservation decreased hepatocyte viability, individual viable frozen/thawed hepatocytes demonstrated clonal replicative potential identical to that of fresh hepatocytes. Even after storage for 32 months in liquid nitrogen, transplanted hepatocytes constituting 0.1% of total adult hepatocyte number could repopulate a mean of 32% of recipient liver parenchyma.ConclusionsThese findings suggest that cryopreserved hepatocytes represent an appropriate source of cells for therapeutic hepatocyte transplantation. GASTROENTEROLOGY 2000;118:390-394
