Characterization of the interaction between Oidium heveae and Arabidopsis thaliana
Characterization of the interaction between Oidium heveae and Arabidopsis thaliana
复制标题
橡胶囊肿与拟南芥之间相互作用的表征
DOI：
10.1111/mpp.12363
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发表时间：
2016-12-01
影响因子：
4.9
通讯作者：
Rong, Wei
中科院分区：
文献类型：
--
作者：
Mei, Shuangshuang;Hou, Shuguo;Rong, Wei
橡胶粉菌（Oidium heveae）是一种专性生物营养型橡胶树病原菌，在世界范围内造成橡胶产量的严重损失。然而，O.橡胶树和橡胶树仍然基本上不为人所知。在这项研究中，我们分离出一个O。HN 1106是一株从人工栽培的橡胶树中分离得到的菌株。海南的巴西花。我们发现O.橡胶树HN 1106以依赖于效应子触发的免疫蛋白EDS 1（增强的疾病易感性1）和PAD 4（植物抗毒素缺陷4）以及依赖于模式植物拟南芥中的水杨酸（SA）的方式触发过敏反应。然而，SA-独立电阻似乎也限制O。heveae感染拟南芥，因为病原体不产生分生孢子上npr 1（pr 1的非表达），npr 2（SA诱导缺陷2）和NahG植物，这表明在SA信号中断。此外，我们还发现胼胝质合成酶PMR 4（Powdery Mildew Resistant 4）能抑制O. heveae HN 1106在侵染初期侵入叶片。阐明拟南芥对O. heveae HN 1106，用该病原菌接种47份不同的拟南芥材料，分析其病害症状和O. heveae HN 1106菌丝生长和分生孢子形成。结果表明，材料Lag 2 -2对O.橡胶树HN 1106。总之，本研究为今后防治O.橡胶树中的橡胶树。
Oidium heveae, an obligate biotrophic pathogen of rubber trees (Hevea brasiliensis), causes significant yield losses of rubber worldwide. However, the molecular mechanisms underlying the interplay between O. heveae and rubber trees remain largely unknown. In this study, we isolated an O. heveae strain, named HN1106, from cultivated H. brasiliensis in Hainan, China. We found that O. heveae HN1106 triggers the hypersensitive response in a manner that depends on the effector-triggered immunity proteins EDS1 (Enhanced Disease Susceptibility 1) and PAD4 (Phytoalexin Deficient 4) and on salicylic acid (SA) in the model plant Arabidopsis thaliana. However, SA-independent resistance also appears to limit O. heveae infection of Arabidopsis, because the pathogen does not produce conidiospores on npr1 (nonexpressor of pr1), sid2 (SA induction deficient 2) and NahG plants, which show disruptions in SA signalling. Furthermore, we found that the callose synthase PMR4 (Powdery Mildew Resistant 4) prevents O. heveae HN1106 penetration into leaves in the early stages of infection. To elucidate the potential mechanism of resistance of Arabidopsis to O. heveae HN1106, we inoculated 47 different Arabidopsis accessions with the pathogen, and analysed the plant disease symptoms and O. heveae HN1106 hyphal growth and conidiospore formation on the leaves. We found that the accession Lag2-2 showed significant susceptibility to O. heveae HN1106. Overall, this study provides a basis for future research aimed at combatting powdery mildew caused by O. heveae in rubber trees.