Cloning and sequence of the human RecA-like gene cDNA.
Cloning and sequence of the human RecA-like gene cDNA.
复制标题
人类 RecA 样基因 cDNA 的克隆和序列。
DOI：
10.1093/nar/21.7.1665
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发表时间：
1993
影响因子：
14.9
通讯作者：
Aizo Matsushiro
中科院分区：
文献类型：
--
作者：
Y. Yoshimura;Takashi Morita;A. Yamamoto;Aizo Matsushiro
酿酒酵母的RAD51基因属于Rad52上位群，已通过对电离辐射的敏感性鉴定出该基因。该基因的突变会导致 X 射线敏感性、无法修复双链 DNA 断裂、自发和诱导有丝分裂重组减少以及减数分裂重组减少。它们还会导致孢子形成不良、活力低以及交配类型转换缺陷 (1)。克隆了酵母 RAD51 cDNA，其基因产物在结构上与大肠杆菌 RecA 蛋白相似，可与单链和双链 DNA 结合，并由 UV 和 y 射线诱导 (2-4)。我们从小鼠睾丸中分离出了酵母RAD51基因的cDNA同源物，并表明酵母RAD51基因的同源物普遍存在于包括人类在内的脊椎动物中（已提交给其他地方）。使用小鼠 Rad5J 同源物的 cDNA 作为探针，我们分离出了
The RAD51 gene of the yeast Saccharomyces cerevisiae belongs to Rad52 epistasis group, which has been identified by sensitivity to ionizing radiation. Mutations in this gene cause X-ray sensitivity, inability to repair double-strand DNA breaks, decreased spontaneous and induced mitotic recombination and decreased meiotic recombination. They also result in poor formation of spores with low viability and deficiencies in matingtype switching (1). The yeast RAD51 cDNA is cloned and its gene product is shown to be structurally similar to E. coli RecA protein, to bind to single and double-stranded DNAs and to be induced by UV and -y-ray (2-4). We have isolated a cDNA homolog of the yeast RAD51 gene from mouse testis and have shown that the homologs of yeast RAD51 gene exist universally in vertebrates including man (submitted to elsewhere). Using the cDNA of a mouse Rad5J homolog as a probe, we have isolated