Molecular survey of Ehrlichia infection in ticks from animals in Yamaguchi Prefecture, Japan
Molecular survey of Ehrlichia infection in ticks from animals in Yamaguchi Prefecture, Japan
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DOI：
10.1016/s0304-4017(01)00470-8
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发表时间：
2001-08-31
影响因子：
2.6
通讯作者：
Brouqui, P
中科院分区：
文献类型：
--
作者：
Inokuma, H;Parola, P;Brouqui, P
共82蜱收集的野生动物和狗在山口县，日本埃立克体感染进行了检查，使用聚合酶链反应（PCR）引物扩增的埃立克体属的大多数成员的DNA。来自日本山口县熊的卵形硬蜱若虫的DNA样品在筛选PCR中呈阳性。随后用两对引物进行PCR扩增，得到16SrRNA基因的一个1431bp的片段，其序列与E. chaffeensis和E.和最近描述的分离自I. muris的埃立克体属种的菌株变体。在日本其他地区也是如此。(C)2001年爱思唯尔科学BN。All rights reserved.
A total of 82 ticks collected from wild animals and dogs in Yamaguchi Prefecture, Japan were examined for Ehrlichia infection by using polymerase chain reaction (PCR) primers that amplify DNA of most members of the genus Ehrlichia. A DNA sample from an Ixodes ovatus nymph from a bear in Yamaguchi Prefecture, Japan, was positive in the screening PCR. Subsequent PCR using two sets of primers yielded a 1431 bp segment of the 16S rRNA gene and the sequence was very similar to those of E. chaffeensis and E. muris, and a strain variant of a recently described Ehrlichia species isolated from I. ovatus in other prefectures of Japan. (C) 2001 Elsevier Science BN. All rights reserved.