Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors
Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors
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DOI：
10.1007/s10616-012-9446-1
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发表时间：
2012-12-01
期刊：
影响因子：
2.2
通讯作者：
Yuk, Inn H.
中科院分区：
文献类型：
--
作者：
Hsu, Wei-Ting;Aulakh, Rigzen P. S.;Yuk, Inn H.
近年来，已经开发了几种自动化规模缩小的生物反应器系统，以提高细胞培养工艺开发的效率。ambr（TM）是一种自动化工作站，可在10-15 mL的工作体积下对一次性搅拌罐生物反应器中的培养物溶解氧和pH值进行单独监测和控制。为了评价ambr（TM）系统，我们比较了四种重组中国仓鼠卵巢细胞系在平行ambr（TM）、2-L台式生物反应器和摇瓶中的补料分批工艺中的性能。ambr（TM）中的培养物在控制环境（温度、溶解氧和pH）和培养性能（生长、活力、葡萄糖、乳酸盐、Na+、渗透压、滴度和产品质量）方面与2 L生物反应器相匹配。然而，摇瓶中的培养物没有显示出与ambr（TM）和2-L生物反应器相当的性能。
In recent years, several automated scale-down bioreactor systems have been developed to increase efficiency in cell culture process development. ambr (TM) is an automated workstation that provides individual monitoring and control of culture dissolved oxygen and pH in single-use, stirred-tank bioreactors at a working volume of 10-15 mL. To evaluate the ambr (TM) system, we compared the performance of four recombinant Chinese hamster ovary cell lines in a fed-batch process in parallel ambr (TM), 2-L bench-top bioreactors, and shake flasks. Cultures in ambr (TM) matched 2-L bioreactors in controlling the environment (temperature, dissolved oxygen, and pH) and in culture performance (growth, viability, glucose, lactate, Na+, osmolality, titer, and product quality). However, cultures in shake flasks did not show comparable performance to the ambr (TM) and 2-L bioreactors.