Dietary accumulation of UV-absorbing mycosporine-like amino acids (MAAs) by the green sea urchin (Strongylocentrotus droebachiensis)
Dietary accumulation of UV-absorbing mycosporine-like amino acids (MAAs) by the green sea urchin (Strongylocentrotus droebachiensis)
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DOI：
10.1007/bf00351037
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发表时间：
1996-02-01
期刊：
影响因子：
2.4
通讯作者：
Shick, JM
中科院分区：
文献类型：
--
作者：
Carroll, AK;Shick, JM
在富含和缺乏MAA的饲料中测定了Droebachiensis（Muller）对吸收紫外线的类菌孢素氨基酸（MAA）的生物积累。个体被喂食红色的Mastocarpus stellatus（Stackhouse），其具有高浓度的MAA shinorine（6.98 nmol mg（-1）干重）和痕量的MAA porphyra-334、asterina-330和palythine，或者棕色的Laminaria saccharina（Lamouroux），其不包含可检测的MAA。在1992年至1993年的9个月期间，自由采食繁殖用的海胆，直到它们再次妊娠。为了监测蓄积，在整个9个月期间，从维持每种饲料的雄性和雌性动物中采集海胆组织和摄入食物样品，并使用高效液相色谱法测定MAA浓度。海胆维持在饮食的hi。星毛乳杆菌的卵巢中shinorine浓度（8.33 nmol mg（-1）干重）比饲喂糖精乳杆菌的高25倍。两个饲养组的睾丸或体细胞组织中的MAA含量均无变化。在实验性的M.星状肌在通过肠道的过程中被去除。先前的研究通过比较动物组织中存在的MAA与消费者在所持有的潜在饮食来推断MAA的饮食积累。这是第一项监测MAA在动物组织中蓄积的研究，并结合具有已知MAA组成的受控饮食。在卵巢中集中的MAA可以为释放到水柱中的卵子提供保护，使其免受太阳紫外线辐射的破坏。
The bioaccumulation of ultraviolet radiation-absorbing mycosporine-like amino acids (MAAs) by Strongylocetrotus droebachiensis (Muller) was determined in specimens maintained on MAA-rich and MAA-deficient diets. Individuals were fed either the red alga Mastocarpus stellatus (Stackhouse), which has a high concentration of the MAA shinorine (6.98 nmol mg(-1) dry wt), and trace amounts of the MAAs porphyra-334, asterina-330 and palythine, or the brown alga Laminaria saccharina (Lamouroux), which contains no detectable MAAs. Reproductively spent urchins were fed ad libitum during a 9 mo period in 1992 to 1993 until they were once again gravid. To monitor accumulation, samples of urchin tissues and ingested food were taken throughout the 9 mo period from males and females maintained on each diet, and the concentrations of MAAs determined using high performance liquid chromatography. Urchins maintained on a diet of hi. stellatus showed an ovarian shinorine concentration (8.33 nmol mg(-1) dry wt) 25 times higher than those fed L.saccharina. There was no change in MAA content of testes or somatic tissues in either feeding group. More than 99% of the MAAs present in an experimental meal of M. stellatus were removed during passage through the gut. Previous studies have inferred dietary accumulation of MAAs by comparing MAAs present in animal tissues with the consumer's potential diet in the held. This is the first study to have monitored MAA accumulation in animal tissues in conjunction with a controlled diet having a known MAA composition. Concentrating MAAs in the ovaries may provide eggs released into the water column with protection from damaging solar ultraviolet radiation.