Phosphate-starvation induced changes in Thiobacillus ferrooxidans.
Phosphate-starvation induced changes in Thiobacillus ferrooxidans.
复制标题
磷酸盐饥饿引起氧化亚铁硫杆菌的变化。
作者：
M. Seeger;C. Jerez
我们分析了嗜酸性趋化性细菌氧化亚铁硫杆菌对磷酸盐饥饿的反应。在没有添加磷酸盐的情况下培养这种细菌，可以诱导细胞显着的丝状化。聚丙烯酰胺凝胶电泳法显示了几种蛋白质在磷酸盐限制下水平升高，以及一些多肽是在这种生长限制下专门合成的。其中一种因缺磷而升高的蛋白质明显是一种酸性磷酸酶，其最适pH约为3.8，分子量约为26 kDa，位于周质中。该蛋白的N末端序列与已知的溶杆菌酶基因和大肠杆菌碱性磷酸酶序列分别有30%和35%的同源性。
We have analysed the response of the acidophilic chemolithotroph Thiobacillus ferrooxidans to phosphate starvation. Cultivation of the bacteria in the absence of added phosphate induced a remarkable filamentation of the cells. Polyacrylamide gel electrophoresis revealed several proteins whose levels increased upon phosphate limitation, as well as some polypeptides that were exclusively synthesized under this growth limitation. One of the proteins whose level increased by the lack of phosphate was apparently an acid phosphatase with a pH optimum of about 3.8, and a molecular mass of 26 kDa, which was located in the periplasm. The N-terminal sequence of a 26 kDa protein derepressed by starvation, which may correspond to the T. ferrooxidans starvation, which may correspond to the T. ferrooxidans phosphatase, showed 30% and 35% identity with the known sequence of Lysobacter enzymogenes and Escherichia coli alkaline phosphatases, respectively.
影响因子：
2.9
作者：
BLAKE, MS;JOHNSTON, KH;GOTSCHLICH, EC
通讯作者：
GOTSCHLICH, EC
DOI：
10.1073/pnas.78.6.3473
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BRADSHAW, RA;CANCEDDA, F;WALSH, KA
通讯作者：
WALSH, KA