Mapping of the SLA complex of miniature swine: Mapping of the SLA gene complex by pulsed field gel electrophoresis
Mapping of the SLA complex of miniature swine: Mapping of the SLA gene complex by pulsed field gel electrophoresis
复制标题
小型猪 SLA 复合物的定位：通过脉冲场凝胶电泳定位 SLA 基因复合物
作者：
Yuanxin Xu;M. Rothschild;C. Warner
The overall order of the regions of the swine major histocompatibility complex (MHC), the SLA complex, was determined by pulsed field gel electrophoresis (PFGE). It was found that the order of the regions is class II-class III-class I. A class I probe hybridized to a 420 kbMlu I and a 420 kbNot I fragment as did a class III probe forC2. None of the class II probes hybridized to these fragments. Thus, linkage of class I to class III was shown. The class IIiC2, Bf, andC4 genes were found to reside in a 190 kbNot I fragment. Linkage of class III and class II genes was shown when both the class IIiC4 and the class IiDR probes hybridized to the same 195 kbSac II and 340 kbNot I fragments. The class I probe did not hybridize to these fragments. The order of the regions, class II-class III-class I, is similar to that of human MHC genes and may have been conserved in evolution so that coordinated expression of MHC genes could be achieved.
通过原位杂交将猪主要组织相容性复合体分配至 7 号染色体。
DOI：
10.1159/000132136
发表时间：
1985
期刊：
Cytogenetics and cell genetics
影响因子：
--
作者：
Rabin,M;Fries,R;Singer,D;Ruddle,FH
通讯作者：
Ruddle,FH