Lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus.
Lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus.
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DOI：
10.1016/0042-6822(86)90195-9
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发表时间：
1986-12
期刊：
影响因子：
3.7
通讯作者：
K. Rushlow;K. Olsen;G. Stiegler;S. Payne;R. Montelaro;C. Issel
中科院分区：
文献类型：
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作者：
K. Rushlow;K. Olsen;G. Stiegler;S. Payne;R. Montelaro;C. Issel
马传染性贫血病毒（EIAV）是逆转录病毒慢病毒亚科的一个成员，它的包膜基因（env）区的核苷酸序列已从一个整合的前病毒DNA克隆中测定，其gagandpolsequences已被报道。该基因全长859个密码子，与已发表的EIAV糖蛋白的生物化学特性一致。EIAV的env基因区域与慢病毒亚科其他成员、维斯纳病毒、人类嗜T淋巴细胞病毒（HTLV-III）或淋巴结病病毒（LAV）的env基因有相当大的结构相似性，但序列同源性可忽略不计。与维斯纳病毒和HTLV-III一样，EIAV的聚合酶（pol）和编码基因不重叠。EIAV在pol-envintergenic区含有两个短的开放阅读框架（ORF），分别为50和66个密码子。然而，与visna病毒和HTLV-III报告的orf Q区不同，EIAV orf均不与相邻polgene的3′端重叠。EIAV基因组还含有第三个短的开放阅读框，由135个密码子组成，完全包含在env基因内，而HTLV-Ⅲ/LAV的3′-orf/orf F基因则延伸到env基因末端之外。这些结果提供了EIAV的envgene区域的详细描述，并描述了慢病毒基因组组织的一些特征，与致癌逆转录病毒的基因组组织形成对比。
The nucleotide sequence of the envelope (env) gene region of equine infectious anemia virus (EIAV), a member of the lentivirus subfamily of retroviruses, has been determined from a clone of integrated proviral DNA for which thegagandpolsequences have been reported previously. Theenvgene is 859 codons in length and the sequence reported here is consistent with the published biochemical properties of EIAV glycoproteins. Theenvgene region of EIAV shares considerable structural similarities but negligible sequence homologies with theenvgenes of other members of the lentivirus subfamily, visna virus, and human T-lymphotropic virus (HTLV-III) or lymphadenopathy virus (LAV). As in visna virus and HTLV-III, the polymerase (pol) andenvgenes of EIAV do not overlap. EIAV contains two short open reading frames (orf) of 50 and 66 codons in thepol-envintergenic region. However, unlike the orf Q regions reported for visna virus and HTLV-III, neither EIAV orf overlaps the 3′ terminus of the adjacentpolgene. The EIAV genome also contains a third short open reading frame of 135 codons which is contained completely within theenvgene, in contrast to the 3′-orf/orf F gene reported for HTLV-III/LAV which extends beyond theenvgene terminus. These results provide a detailed description of theenvgene region of EIAV and describe a number of characteristic features of genomic organization in lentiviruses which contrast with the genomic organization of oncogenic retroviruses.