Documenting the epidemiologic patterns of polyomaviruses in human populations by studying their presence in urban sewage
Documenting the epidemiologic patterns of polyomaviruses in human populations by studying their presence in urban sewage
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DOI：
10.1128/aem.66.1.238-245.2000
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发表时间：
2000-01-01
影响因子：
4.4
通讯作者：
Girones, R
中科院分区：
文献类型：
--
作者：
Bofill-Mas, S;Pina, S;Girones, R
这是第一次描述，据我们所知，人类多瘤病毒和猿猴病毒40（SV 40）在城市污水中的分布，使用巢式PCR程序，我们报告检测人类多瘤病毒JC病毒（JCV）和BR病毒（BKV），但不是SV 40在很高比例的城市污水样品中获得广泛不同的地理区域在欧洲和非洲。在总共分析的28份样本中，26份检测到JCV，22份检测到BKV，无SV 40阳性。所有地理区域均显示这些病毒的高流行率，巴塞罗那（西班牙）和南希（法国）的JC病毒颗粒平均估计值约为10（3）/ml，比勒陀利亚（南非）和于默奥（瑞典）约为10（2）/ml，比勒陀利亚和巴塞罗那的BK病毒颗粒平均值约为10（2）/ml，南希和于默奥约为10（1）/ml。这与作为对照评价的人腺病毒的估计平均值10（2）至10（3）相比。还提供了来自一些样品的扩增DNA的核苷酸序列分析，并代表了这些样品中最丰富的JC和BK病毒株的序列。还在系统发育研究中分析了检测到的JCV的核苷酸序列，并在调控区进行了基因组表征。这项研究表明，人类多瘤病毒在不同地理区域的污水中以高浓度传播，并存在于受污染的环境中。在环境中检测到的JCV的频率和浓度以及所描述的动物宿主的缺乏表明，JCV可能是有用的人为来源的粪便污染的标志物。这些结果也支持了先前描述的观点，即JCV菌株与所研究人群的种族来源密切相关。所应用的程序也应该是有用的，在未来的研究人群的病毒排泄模式，并作为一种工具，在流行病学研究中的变化，在特定的病毒病原体的流行检测。
This is the first description, to our knowledge, of the distribution of human polyomavirus and simian virus 40 (SV40) in urban sewage, Using a nested-PCR procedure, we report the detection of human polyomaviruses JC virus (JCV) and BR virus (BKV) but not SV40 in a high percentage of urban sewage samples obtained from widely divergent geographical areas in Europe and Africa. For a total of 28 samples analyzed, JCV was detected in 26, BKV was detected in 22, and none was positive for SV40. All geographical areas showed a high prevalence of these viruses with mean estimated values of JC viral particles per mi on the order of 10(3) in Barcelona (Spain) and Nancy (France) and 10(2) in Pretoria (South Africa) and Umea (Sweden) and mean values of BK viral particles on the order of 10(2) in Pretoria and Barcelona and 10(1) in Nancy and Umea. This compares with estimated mean values of 10(2) to 10(3) for human adenovirus that was evaluated as a control. Nucleotide sequence analysis of the amplified DNA from some of the samples is also presented and represents the sequence of the most abundant JC and BK viral strains in these samples. The nucleotide sequence of the JCV detected was also analyzed in a phylogenetic study and for genomic characterization in the regulatory region. This study has shown that human polyomaviruses are spread in high concentrations in the sewage of different geographical areas and are present in contaminated environments. The frequency and concentration of JCV detected in the environment and the absence of described animal hosts suggest that JCV may be useful as a marker for fecal pollution of anthropogenic origin. The results also support the idea previously described that the strains of JCV are closely related to the ethnic origin of the population studied. The procedure applied should also be useful in future studies of population patterns of viral excretion and as a tool in epidemiological studies for the detection of changes in the prevalence of specific viral pathogens.