One-Cell Analysis as a Technique for True Single-Cell Analysis of Organelles in Breast Tumor and Adjacent Normal Tissue to Profile Fatty Acid Composition of Triglyceride Species
One-Cell Analysis as a Technique for True Single-Cell Analysis of Organelles in Breast Tumor and Adjacent Normal Tissue to Profile Fatty Acid Composition of Triglyceride Species
复制标题
单细胞分析作为一种技术，对乳腺肿瘤和邻近正常组织中的细胞器进行真正的单细胞分析，以分析甘油三酯种类的脂肪酸组成
DOI：
10.6000/1927-7229.2016.05.02.1
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
G. Verbeck
中科院分区：
文献类型：
--
作者：
Jason S. Hamilton;G. Verbeck
乳腺癌是在正常脂肪细胞富含脂肪的环境中发生的，已知正常脂肪细胞通过一种未知的方法将脂质从正常细胞转移到肿瘤细胞，从而有助于肿瘤的进展。围绕乳腺肿瘤和邻近正常组织的脂质分析进行了大量研究，以确定脂质组的变化，从而了解脂质在癌症进展中的作用。理想情况下，单细胞分析方法与质谱相结合，保留空间信息是最适合这项工作的。然而，许多单细胞分析方法不能在保持空间信息的同时对完整的脂质进行亚细胞分析。单细胞分析是一种真正的单细胞技术，可以精确地从完整组织中提取单个细胞器，同时不干扰或破坏相邻细胞。该方法采用纳米操作耦合纳米电喷雾电离质谱法从单个细胞中提取和分析单个细胞器。本文展示了对两组不同的乳腺肿瘤和正常邻近组织的单一脂质体进行分析，以阐明使用单细胞分析耦合串联质谱法甘油三酯的脂肪酸组成。结果，鉴定出肿瘤组织特有的13种脂肪酸，其中5种在一组组织中，8种在另一组组织中。
Breast cancer develops in an adipose rich environment of normal adipocytes that are known to aid in tumor progression through an unknown method of lipid transfer from normal cells to tumor cells. Much research is built around lipid analysis of breast tumor and adjacent normal tissues to identify variations in the lipidome to gain an understanding of the role lipids play in progressing cancer. Ideally, single-cell analysis methods coupled to mass spectrometry that retain spatial information are best suited for this endeavor. However, many single-cell analysis methods are not capable of subcellular analysis of intact lipids while maintaining spatial information. One-Cell analysis is a true single-cell technique with the precision to extract single organelles from intact tissues while not interfering or disrupting adjacent cells. This method is used to extract and analyze single organelles from individual cells using nanomanipulation coupled to nanoelectrospray ionization mass spectrometry. Presented here is a demonstration of the analysis of single lipid bodies from two different sets of breast tumor and normal adjacent tissues to elucidate the fatty acid composition of triglycerides using One-Cell analysis coupled to tandem mass spectrometry. As a result, thirteen fatty acid species unique to the tumor tissues were identified, five in one set of tissues and eight in the other set.
影响因子：
7.7
作者：
Rubakhin, Stanislav S.;Lanni, Eric J.;Sweedler, Jonathan V.
通讯作者：
Sweedler, Jonathan V.
DOI：
10.1016/j.bbalip.2013.02.010
发表时间：
2013-10
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Nieman KM;Romero IL;Van Houten B;Lengyel E
通讯作者：
Lengyel E
影响因子：
2.1
作者：
Robinson, Michael A.;Graham, Daniel J.;Gamble, Lara J.
通讯作者：
Gamble, Lara J.