Three dimensional spheroids formation platform using micro-rotation flow
Three dimensional spheroids formation platform using micro-rotation flow
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DOI：
10.1109/mhs.2008.4752450
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
H. Ota;K. Deguchi;S. Kita;R. Yamamoto;N. Miki
中科院分区：
文献类型：
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作者：
H. Ota;K. Deguchi;S. Kita;R. Yamamoto;N. Miki
肝细胞负责肝脏的代谢和解毒过程。在分离的细胞形成组织的过程中，它们增加了细胞密度，获得了肝脏特异性功能、新的蛋白表达和细胞信号。因此，基于组织的体外研究是非常必要的。我们开发了一种MEMS平台，通过对细胞的显微荧光处理来形成3D球体。微流控平台由微刻制的PDMS腔体和循环系统组成。利用FLUENT软件对燃烧室内的流动进行了数值模拟，发现当燃烧室高度大于通道高度的4倍时，会产生微旋转流动。利用新设计的微腔，利用荧光微珠对旋转流动进行了实验观察。提出的基于MEMS的三维球体形成平台很容易开创生物学领域的技术创新，成为细胞研究和组织研究之间的桥梁。
Hepatocytes are responsible for metabolic and detoxification processes in the liver. During the tissue formation from isolated cells, they increase the cell density and acquire their liver-specific functions, new protein expression and cell signaling. Hence, tissue-based in vitro studies are strongly demanded. We develop a MEMS platform which forms 3D spheroids by microfluodic handing of cells. The microfludic platform is composed of PDMS chamber fabricated by microlithography and a circulation system. We simulated the flow in the chamber by FLUENT and found that micro-rotation flow was generated when the chamber height was four times greater than the channel height. The rotation flow was experimentally observed using fluorescent microbeads with the newly designed micro-chamber. The proposed MEMS-based 3D-spheroids formation platform is readily to create a technological innovation in biology as a bridge between cell researches and tissue researches.