IDENTIFICATION OF A NEW VENEZUELAN EQUINE ENCEPHALITIS-VIRUS FROM BRAZIL
IDENTIFICATION OF A NEW VENEZUELAN EQUINE ENCEPHALITIS-VIRUS FROM BRAZIL
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DOI：
10.4269/ajtmh.1982.31.1260
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发表时间：
1982-01-01
影响因子：
3.3
通讯作者：
FRANCY, DB
中科院分区：
文献类型：
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作者：
CALISHER, CH;KINNEY, RM;FRANCY, DB
从巴西南部分离到两株委内瑞拉马脑炎（VEE）复合病毒，对6-8周龄小鼠和短毛豚鼠无毒力。它们在血清学上被证明是一种新发现的I亚型变异体。双向聚丙烯酰胺凝胶电泳（PAGE）的RNase T1酶的病毒RNA显示相当大的同源性之间的基因组的新的变体原型和变体IA。通过不连续十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）观察到三种结构蛋白。虽然最近分离的最小的蛋白质迁移与其他亚型I病毒的衣壳蛋白，较大的结构蛋白的新变种不同MW从E1和E2包膜糖蛋白的其他亚型I的变种。用金黄色葡萄球菌V8蛋白酶消化解离的病毒粒子后进行SDS-PAGE，新分离株产生的肽片段模式彼此相同，但与其他I亚型病毒的模式不同。由于这2个分离株来自库蚊（黑锥虫）种蚊子和蝙蝠（黑锥虫），因此推测这是一种地方流行的VEE病毒变体，建议将其分类为IF。
Two strains of a recently isolated Venezuelan equine encephalitis (VEE) complex virus from southern Brazil, avirulent for 6-8 wk old mice and short-haired guinea pigs, were characterized by biologic, serologic and biochemical means. They were shown serologically to represent a single, newly recognized variant of subtype I. Two-dimensional polyacrylamide gel electrophoresis (PAGE) of RNase T1 digests of viral RNA showed considerable homology between the genomes of the new variant prototype and variant IA. Three structural proteins were visualized by discontinuous sodium dodecyl sulfate-PAGE (SDS-PAGE). Although the smallest protein of both recent isolates migrates with the capsid proteins of other subtype I viruses, the larger structural proteins of the new variants differ in MW from the E1 and E2 envelope glycoproteins of the other subtype I variants. The new isolates produced peptide fragment patterns that were identical to each other, but different from the patterns of other subtype I viruses, following SDS-PAGE of dissociated virions digested with Staphylococcus aureus V8 protease. Since these 2 isolates were from Culex (Melanoconion) species mosquitoes and from a bat (Carollia perspicillata), it is postulated that this is an enzootic VEE virus variant for which the classification IF is suggested.