The frequency of cells secreting interferon-gamma and interleukin-4, -5, and -10 in the blood and duodenal mucosa of children with cow's milk hypersensitivity
The frequency of cells secreting interferon-gamma and interleukin-4, -5, and -10 in the blood and duodenal mucosa of children with cow's milk hypersensitivity
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DOI：
10.1203/00006450-199711000-00014
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发表时间：
1997-11-01
影响因子：
3.6
通讯作者：
MacDonald, TT
中科院分区：
文献类型：
--
作者：
Hauer, AC;Breese, EJ;MacDonald, TT
酶联免疫吸附点 (ELISPOT) 已用于分析从患有牛奶敏感性肠病 (CMSE)、牛奶过敏 (CMA) 和年龄匹配对照儿童的血液中分离出的单核细胞中自发分泌干扰素 γ (INF-γ)、IL-4、IL-5 或 IL-10 的细胞频率。此外，还对 CMSE 患者和对照受试者的十二指肠固有层淋巴细胞的细胞因子谱进行了比较。在血液中，自发性细胞因子分泌细胞并不常见，但与对照受试者相比，CMSE 和 CMA 儿童的 IFN-γ、IL-4、IL-5 和 IL-10 ELISPOT 显着增加。与患有 CMSE 的儿童相比，患有 CMA 的儿童血液中的 IL-4 ELISPOT 含量明显更高。固有层中自发细胞因子分泌细胞的频率比血液中高。与对照组相比，CMSE 中 ELISPOT 的 IFN-γ 和 IL-4 显着增加。与对照组相比，CMSE 中的 IL-5 ELISPOT 没有变化，IL-10 ELISPOT 有所减少。这些结果表明，牛奶过敏儿童血液中 Th-1 和 Th-2 型细胞因子分泌细胞普遍增强，尽管 CMA 血液中 IL-4 分泌细胞增加可能与该疾病是 IgE 介导的事实相关。在固有层中，与对照受试者相比，CMSE 中的 IFN-γ 和 IL-4 分泌细胞也有所增强；然而，分泌 IFN-γ 的细胞数量比分泌 IL-4 的细胞多 10 倍，显示在对照和 CMSE 患者中 Th-1 型反应占主导地位。
Enzyme-linked immunoabsorbant spots (ELISPOTs) have been used to analyze the frequency of cells spontaneously secreting interferon-gamma (INF-gamma), IL-4, IL-5, or IL-10 in mononuclear cells isolated from the blood of children with cow's milk-sensitive enteropathy (CMSE), cow's milk allergy (CMA), and age-matched controls. In addition, cytokine profiles of duodenal lamina propria lymphocytes were compared in patients with CMSE and control subjects. In blood, spontaneous cytokine-secreting cells were uncommon, but there was significantly increased IFN-gamma, IL-4, IL-5, and IL-10 ELISPOTs in children with CMSE and CMA compared with control subjects. IL-4 ELISPOTs were significantly greater in the blood of children with CMA compared with those with CMSE. In the lamina propria the frequencies of spontaneous cytokine-secreting cells were high compared with that in blood. Significantly increased ELISPOTs for IFN-gamma and IL-4 were found in CMSE compared with controls. IL-5 ELISPOTs were unchanged, and IL-10 ELISPOTs were reduced in CMSE compared with controls. These results show a general enhancement of Th-1 and Th-2-type cytokine-secreting cells in the blood of children with cow's milk hypersensitivity, although the increased IL-4-secreting cells in blood in CMA may be of relevance in view of the fact that this disease is IgE-mediated. In the lamina propria, there is also enhancement of IFN-gamma- and IL-4-secreting cells in CMSE compared with control subjects; however, cells secreting IFN-gamma are 10 times more numerous than cells secreting IL-4, showing a dominance of Th-1-type responses in both controls and CMSE patients.