Engineering K+ channels using semisynthesis.
Engineering K+ channels using semisynthesis.
复制标题
使用半合成工程 K 通道。
DOI：
10.1007/978-1-62703-345-9_1
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Valiyaveetil,FrancisI
中科院分区：
文献类型：
--
作者：
Komarov,AlexanderG;Costantino,CoreyA;Valiyaveetil,FrancisI
Potassium channels conduct K+ions selectively and at very high rates. Central to the function of K+channels is a structural unit called the selectivity filter. In the selectivity filter, a row of four K+binding sites are created using mainly the backbone carbonyl oxygen atoms. Due to the involvement of the protein backbone, site-directed mutagenesis is of limited utility in investigating the selectivity filter. In order to overcome this limitation, we have developed a semisynthetic approach, which permits the use of chemical synthesis to manipulate the selectivity filter. In this chapter, we describe the protocols that we have developed for the semisynthesis of the K+channel, KcsA. We anticipate that the protocols described in this chapter will also be applicable for the semisynthesis of other integral membrane proteins of interest.
