Temporal Changes in Myeloid Cells in the Cervix during Pregnancy and Parturition
Temporal Changes in Myeloid Cells in the Cervix during Pregnancy and Parturition
复制标题
DOI：
10.4049/jimmunol.0803138
复制
发表时间：
2009-03-01
影响因子：
4.4
通讯作者：
Mahendroo, Mala S.
中科院分区：
文献类型：
--
作者：
Timmons, Brenda C.;Fairhurst, Anna-Marie;Mahendroo, Mala S.
在美国，早产发生率为12.7%，是出生后第一年胎儿发病的主要原因，也是以后健康问题的原因。阐明控制宫颈重塑的机制对于开发降低早产发生率的治疗方法至关重要。子宫颈细胞外基质必须在分娩过程中被破坏，以允许分娩，然后在产后迅速修复。白细胞在出生前后浸润宫颈，并被认为在宫颈成熟期间通过释放蛋白水解酶来调节基质重塑。在本研究中，流式细胞术和细胞分选用于确定免疫细胞在分娩前、分娩中和分娩后宫颈基质重塑中的作用。评估骨髓细胞分化和活化的标志物以确定表型和功能。组织单核细胞和嗜酸性粒细胞增加，在子宫颈出生前在孕甾酮调节的方式，而巨噬细胞的数量不变。中性粒细胞在产后增加。在分娩过程中或产后不久，Csfr 1和M2巨噬细胞交替活化标志物的mRNA表达增加表明M2巨噬细胞在产后组织修复中的功能。颈髓细胞数量的变化不反映在外周血中。这些数据与我们以前的研究沿着表明，骨髓来源的细胞并不协调出生前启动宫颈成熟所需的过程。此外，具有不同表型（M1和M2）的巨噬细胞存在于子宫颈中，并且最有可能参与组织的产后修复。免疫学杂志，2009，182：2700 - 2707.
Preterm birth occurs at a rate of 12.7% in the U.S. and is the primary cause of fetal morbidity in the first year of life as well as the cause of later health problems. Elucidation of mechanisms controlling cervical remodeling is critical for development of therapies to reduce the incidence of prematurity. The cervical extracellular matrix must be disorganized during labor to allow birth, followed by a rapid repair postpartum. Leukocytes infiltrate the cervix before and after birth and are proposed to regulate matrix remodeling during cervical ripening via release of proteolytic enzymes. In the current study, flow cytometry and cell sorting were used to determine the role of immune cells in cervical matrix remodeling before, during, and after parturition. Markers of myeloid cell differentiation and activation were assessed to define phenotype and function. Tissue monocytes and eosinophils increased in the cervix before birth in a progesterone-regulated fashion, whereas macrophage numbers were unchanged. Neutrophils increased in the postpartum period. Increased mRNA expression of Csfr1 and markers of alternatively activated M2 macrophages during labor or shortly postpartum suggest a function of M2 macrophages in postpartum tissue repair. Changes in cervical myeloid cell numbers are not reflected in the peripheral blood. These data along with our previous studies suggest that myeloid-derived cells do not orchestrate processes required for initiation of cervical ripening before birth. Additionally, macrophages with diverse phenotypes (M1 and M2) are present in the cervix and are most likely involved in the postpartum repair of tissue. The Journal of Immunology, 2009, 182: 2700-2707.