Eotaxin increases the expression of CD11b/CD18 and adhesion properties in IL5, but not fMLP-prestimulated human peripheral blood eosinophils
Eotaxin increases the expression of CD11b/CD18 and adhesion properties in IL5, but not fMLP-prestimulated human peripheral blood eosinophils
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DOI：
10.1023/a:1022379821130
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发表时间：
1998-04-01
期刊：
影响因子：
5.1
通讯作者：
Halldén, G
中科院分区：
文献类型：
--
作者：
Lundahl, J;Moshfegh, A;Halldén, G
据称，嗜酸性粒细胞选择性募集到炎症部位是通过细胞因子、趋化因子和粘附分子的调节来控制的。在动物模型中，嗜酸性粒细胞趋化因子被认为是一种有效的趋化因子，因为它与白细胞介素 5 协同作用，在过敏原激发后诱导选择性趋化和嗜酸性粒细胞浸润至肺组织。我们研究了嗜酸性粒细胞趋化因子对人外周血嗜酸性粒细胞 CD11b/CD18 表达和基质蛋白纤连蛋白粘附特性的体外影响。我们没有发现嗜酸性粒细胞趋化因子本身对 CD11b/CD18 表达有任何影响，无论是对健康受试者的嗜酸性粒细胞还是无症状花粉相关哮喘患者的嗜酸性粒细胞。然而，eotaxin 显着上调 CD11b/CD18 的定量水平，并增加与 IL-5 体外预孵育的健康受试者的嗜酸性粒细胞中对纤连蛋白的粘附，但与 fMLP 预孵育的嗜酸性粒细胞中则不然。此外，哮喘患者体内过敏原吸入激发后 24 小时收获的嗜酸性粒细胞，在体外仅与嗜酸细胞活化趋化因子一起孵育后，CD11b/CD18 上调。
A selective recruitment of eosinophils to sites of inflammation is claimed to be controlled by regulation of cytokines, chemokines and adhesion molecules. In animal models, eotaxin has been suggested to be a potent chemokine since it in cooperation with interleukin-5 induce selective chemotaxis and infiltration of eosinophils to lung tissue after an allergen provocation. We have investigated the in vitro effect of eotaxin on human peripheral blood eosinophils with respect to CD11b/CD18 expression and adhesion properties to the matrix protein fibronectin. We did not find any effect of eotaxin per se on CD11b/CD18 expression, neither on eosinophils from healthy subjects nor from patients with asymptomatic pollen related asthma. However, eotaxin significantly upregulated the quantitative level of CD11b/CD18 and increased the adhesion to fibronectin in eosinophils from healthy subjects preincubated in vitro with interleukin-5, but not in eosinophils preincubated with fMLP. Moreover, eosinophils harvested 24 hours after an in vivo allergen inhalation provocation in asthmatics, upregulated CD11b/CD18 after in vitro incubation with eotaxin alone.