Fertilization potential and polyspermy prevention in the egg of the nemertean, Cerebratulus lacteus.
Fertilization potential and polyspermy prevention in the egg of the nemertean, Cerebratulus lacteus.
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纽虫、乳脑小脑虫卵的受精潜力和多受精预防。
DOI：
10.1002/jez.1402360107
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Tucker,RP
中科院分区：
文献类型：
--
作者：
Kline,D;Jaffe,LA;Tucker,RP
我们研究了纽虫Cerebratulus卵的电特性，并发现了电介导的多精受精阻滞在受精后约1小时内运作的证据。受精时，在天然或人工海水中，膜电位从约-66 mV的静息水平转变为约+43 mV的峰值，在大多数情况下保持大于0 mV超过1小时。前30 min的平均电位为+22 ± 8 mV（SD，n = 12）。当外部Na+浓度从486 mM降至51 mM（胆碱替代）时，受精电位振幅降低;前30 min的平均电位为−27 ± 21 mV（SD，n = 5）。在51 mM Na+海水中授精的卵子变成多精，表明多精预防依赖于电介导的机制。电阻断需要约60分钟，因为在此期间转移到51 mM Na+海水中会导致多精受精。在受精后的第一个小时内，卵子也会产生永久性的非电性阻滞;转移到低Na+海水后导致的多精受精程度随着时间的推移而逐渐降低。永久性的阻塞似乎是在卵质膜或糖萼的水平上，因为卵被膜不是精子穿透的障碍，它的去除也不会诱导多精受精。电子显微镜观察表明，受精后卵细胞外膜、质膜和皮层的形态无明显变化。
We investigated the electrical properties of the egg of the nemertean wormCerebratulus, and found evidence that an electrically‐mediated polyspermy block operates for a period of about 1 hr after fertilization. At fertilization, in natural or artificial sea water, the membrane potential shifts from its resting level of about −66 mV to a peak of about +43 mV, and in most cases remains greater than 0 mV for more than 1 hr. The average potential during the first 30 min is +22 ± 8 mV (SD, n = 12). When the external Na+concentration is reduced from 486 to 51 mM (choline substituted) the fertilization potential amplitude is reduced; the average potential during the first 30 min is −27 ± 21 mV (SD, n = 5). Eggs inseminated in 51 mM Na+sea water become polyspermic, indicating that polyspermy prevention depends on an electrically‐mediated mechanism. The electrical block is required for about 60 min, since transfer to 51 mM Na+sea water during this period results in polyspermy. During the first hour following fertilization, the egg is also developing a permanent, nonelectrical block; the degree of polyspermy which results upon transfer to low Na+sea water decreases progressively with time. The permanent block appears to be at the level of the egg plasma membrane or glycocalyx, since the egg envelope is not a barrier to sperm penetration, nor does its removal induce polyspermy. Electron micrographs show no obvious changes in the morphology of the extracellular layers, plasma membrane or cortex of the egg after fertilization.
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DOI：
--
发表时间：
1985
期刊：
影响因子：
--
作者：
J. M. Turbeville;E. Ruppert
通讯作者：
E. Ruppert
阻碍毛鳍龙属的多精受精。
DOI：
10.1002/jez.1402330213
发表时间：
1985
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
Eckberg,WR;Anderson,WA
通讯作者：
Anderson,WA
影响因子：
5.5
作者：
KOZUKA, M;TAKAHASHI, K
通讯作者：
TAKAHASHI, K
影响因子：
2.7
作者：
GREY, RD;BASTIANI, MJ;SCHERTEL, ER
通讯作者：
SCHERTEL, ER
DOI：
10.1073/pnas.81.5.1436
发表时间：
1984
影响因子：
11.1
作者：
S. Shen;R. Steinhardt
通讯作者：
R. Steinhardt