p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13.
p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13.
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DOI：
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发表时间：
1998
期刊：
影响因子：
56.9
通讯作者：
T. Kozasa;X. Jiang;M. Hart;P. M. Sternweis;W. Singer;A. Gilman;G. Bollag;P. Sternweis
中科院分区：
文献类型：
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作者：
T. Kozasa;X. Jiang;M. Hart;P. M. Sternweis;W. Singer;A. Gilman;G. Bollag;P. Sternweis
G蛋白信号转导调节因子(RGS)家族的成员刺激某些异源三聚体鸟嘌呤核苷酸结合蛋白(G蛋白)的α亚基的内源性鸟苷三磷酸酶(GTPase)活性。Rho的鸟嘌呤核苷酸交换因子p115的氨基酸末端区域与RGS蛋白相似。重组p115重组蛋白和含有p115氨基末端的融合蛋白对G蛋白G12和G13的α亚基具有GTPase激活活性，而对Galpha蛋白的Gs、Gi或Gq亚家族成员无特异性。这种全环基金可能在G13和G12对Rho蛋白的调节中起中介作用。
Members of the regulators of G protein signaling (RGS) family stimulate the intrinsic guanosine triphosphatase (GTPase) activity of the alpha subunits of certain heterotrimeric guanine nucleotide-binding proteins (G proteins). The guanine nucleotide exchange factor (GEF) for Rho, p115 RhoGEF, has an amino-terminal region with similarity to RGS proteins. Recombinant p115 RhoGEF and a fusion protein containing the amino terminus of p115 had specific activity as GTPase activating proteins toward the alpha subunits of the G proteins G12 and G13, but not toward members of the Gs, Gi, or Gq subfamilies of Galpha proteins. This GEF may act as an intermediary in the regulation of Rho proteins by G13 and G12.