Identification of quantitative trait loci controlling cold tolerance at the reproductive stage in Yunnan landrace of rice, Kunmingxiaobaigu
Identification of quantitative trait loci controlling cold tolerance at the reproductive stage in Yunnan landrace of rice, Kunmingxiaobaigu
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DOI：
10.1270/jsbbs.54.253
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发表时间：
2004-09-01
期刊：
影响因子：
2.4
通讯作者：
Zhang, DP
中科院分区：
文献类型：
--
作者：
Dai, LY;Lin, XH;Zhang, DP
本研究以云南地方品种昆明小白谷为材料，利用DNA分子标记技术对水稻生殖期耐冷性进行了QTL定位。以昆明小白谷与日本品种十和田杂交的F-2群体为材料，构建了一张包含16个连锁群、122个标记的连锁图，总长度为1354.4cM。通过区间作图法在F-2群体中检测到9个QTL。9个QTL分布在第1、3、4、6、7、10和12染色体上。这9个QTL的总贡献率为54.2%，单个QTL的贡献率为5.0%~ 37.8%。在F-3群体中，通过单标记分析，分别检测到位于第3、6和7染色体上的4个QTL（2个QTL），分别命名为qRCT 3、qRCT 6a、qRCT 6 b和qRCT 7。其中qRCT 3、qRCT 6a和qRCT 6 b为首次报道。QTL qRCT 7表现出最大的效应，达到20.6%。
This study focused on a Yunnan landrace of rice, Kunmingxiaobaigu, to identify quantitative trait loci (QTLs) controlling cold tolerance at the reproductive stage using DNA markers. A linkage map with 16 linkage groups covering 1,354.4 cM with a total of 122 markers was constructed based on an F-2 population consisting of 250 individuals of a cross between Kunmingxiaobaigu and a Japanese variety, Towada. Nine QTLs were detected by interval mapping in the F-2 population. The nine QTLs were distributed on chromosomes 1 (2 QTLs), 3, 4, 6 (2 QTLs), 7, 10 and 12. The total variance explained by these nine QTLs was 54.2%, while the variance explained by a single QTL ranged from 5.0% to 37.8%. The validity of four QTLs on chromosomes 3, 6 (2 QTLs) and 7 was confirmed by single-marker analysis in the F-3 population and they were designated as qRCT3, qRCT6a, qRCT6b and qRCT7, respectively. The QTLs qRCT3, qRCT6a and qRCT6b were reported here for the first time. The QTL qRCT7 displayed the largest effect, reaching a value of 20.6%.