Nod1, Nod2 and Nalp3 receptors, new potential targets in treatment of allergic rhinitis?
Nod1, Nod2 and Nalp3 receptors, new potential targets in treatment of allergic rhinitis?
复制标题
DOI：
10.1111/j.1398-9995.2009.02315.x
复制
发表时间：
2010-10-01
期刊：
影响因子：
12.4
通讯作者：
Cardell, L. O.
中科院分区：
文献类型：
--
作者：
Bogefors, J.;Rydberg, C.;Cardell, L. O.
背景：近年来出现了一组新的模式识别受体，即核苷酸结合寡聚化结构域样受体。它们被过敏原或微生物激活，引发炎症反应。NLR在人类airways. Aim的知识是有限的：研究的目的：调查存在的NLR在人的鼻子的健康人和间歇性变应性鼻炎患者以外，并在花粉season.Methods：Nod 1，Nod 2，Nalp 3在鼻活检组织中的表达，用实时荧光定量PCR和免疫组化。培养的原代人鼻上皮细胞（HNECs）进行了分析，使用实时RT-PCR和流式细胞仪，以进一步验证NLR的存在下，在epithelium.Results：免疫组化分析显示存在的Nod 1，Nod 2，Nalp 3在鼻上皮。这在培养的HNEC中得到证实。在花粉季节，患有症状性变应性鼻炎的患者表现出较低的Nod 1和Nalp 3 mRNA水平比对照组和患者。Nod 2的表达被发现在所有的标本测试，但没有显着差异被视为之间的三groups.Conclusion：Nod 1，Nod 2和Nalp 3受体被发现存在于人的鼻子。变应性鼻炎患者花粉季节Nod 1和Nalp 3表达下调。这为炎症性气道疾病开辟了新的见解和新的治疗策略。
P>Background:Recently, a new set of pattern-recognition receptors, the nucleotide-binding oligomerization domain (Nod)-like receptors (NLRs), have emerged. Their activation, either by allergens or microbes, triggers an inflammatory response. The knowledge about NLRs in human airways is limited.Aim of the study:To investigate presence of NLRs in the human nose of healthy individuals and patients with intermittent allergic rhinitis outside and during pollen season.Methods:The expression of Nod1, Nod2, and Nalp3 in nasal biopsies was determined with real-time RT-PCR and immunohistochemistry. Cultured primary human nasal epithelial cells (HNECs) were analyzed using real-time RT-PCR and flow cytometry to further verify the presence of NLRs in the epithelium.Results:Immunohistochemical analysis revealed presence of Nod1, Nod2, and Nalp3 in the nasal epithelium. This was corroborated in cultured HNECs. Patients suffering from symptomatic allergic rhinitis exhibited lower Nod1 and Nalp3 mRNA levels than both controls and patients during pollen season. Nod2 expression was found in all specimens tested, but no differences were seen between the three groups.Conclusion:Nod1, Nod2, and Nalp3 receptors were found to be present in the human nose. The expression of Nod1 and Nalp3 were down-regulated during pollen season among patients with allergic rhinitis. This opens up for new insights and novel therapeutic strategies in inflammatory airway disease.