The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis.
The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis.
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DOI：
10.1056/nejm199001183220307
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发表时间：
1990-01
期刊：
影响因子：
--
通讯作者：
B. Eisenstein
中科院分区：
文献类型：
--
作者：
B. Eisenstein
分子遗传学的发展，作为一个独立的领域和广泛用于生物学研究的技术体系，对医学研究产生了深远的影响。受益者包括基础科学的每一个学科，至少间接地包括大多数临床和应用医学学科。在过去几十年中，可以确定某些技术里程碑，这些里程碑在该领域的进展中特别重要。一个是限制性内切核酸酶的发现，它与DNA连接和转化程序的发展一起，导致了克隆和繁殖任何生物体基因的能力。. .
THE development of molecular genetics, both as a self-contained field and as a body of techniques broadly useful in biologic investigation, has had a profound influence on medical research. The beneficiaries include every discipline in basic science and, at least indirectly, most clinical and applied medical disciplines. Certain technical milestones can be identified over the past several decades that have been particularly important in the progress of the field. One is the discovery of restriction endonucleases, which together with the development of DNA ligation and transformation procedures, led to the ability to clone and thus propagate genes of any organism . . .