Drosophila ERCC1 is required for a subset of MEI-9-dependent meiotic crossovers
Drosophila ERCC1 is required for a subset of MEI-9-dependent meiotic crossovers
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DOI：
10.1534/genetics.104.036178
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发表时间：
2005-08-01
期刊：
影响因子：
3.3
通讯作者：
Sekelsky, J
中科院分区：
文献类型：
--
作者：
Radford, SJ;Goley, E;Sekelsky, J
果蝇MEI-9是核苷酸切除修复（NER）所需的DNA结构特异性内切酶的催化亚基。这种核酸内切酶在NER过程中的酶活性需要第二个非催化亚基ERCC 1的存在。除了其在NER中的作用之外，MEI-9还需要产生大多数减数分裂交换。为了更好地了解MEI-9在交叉形成中的作用，我们在这里报告了果蝇Ercc 1基因的特征。我们通过同源基因打靶创建了Ercc 1突变体。我们发现，Ercc 1突变体是相同的梅-9突变体的DNA损伤剂的敏感性，但有一个不太严重的减数分裂交叉的数量减少。MEI-9蛋白水平在Ercc 1突变体中降低;然而，MEI-9的过表达不足以恢复Ercc 1突变体中的减数分裂交换。我们的结论是，MEI-9可以产生一些减数分裂交换在ERCC 1独立的方式。
Drosophila MEI-9 is the catalytic subunit of a DNA structure-specific endonuclease required for nucleotide excision repair (NER). The enzymatic activity of this endonuclease during NER requires the presence of a second, noncatalytic subunit called ERCC1. In addition to its role in NER, MEI-9 is required for the generation of most meiotic crossovers. To better understand the role of MEI-9 in crossover formation, we report here the characterization of the Drosophila Ercc1 gene. We created an Ercc1 mutant through homologous gene targeting. We find that Ercc1 mutants are identical to mei-9 mutants in sensitivity to DNA-damaging agents, but have a less severe reduction in the number of meiotic crossovers. MEI-9 protein levels are reduced in Ercc1 mutants; however, overexpression of MEI-9 is not sufficient to restore meiotic crossing over in Ercc1 mutants. We conclude that MEI-9 can generate some meiotic crossovers in an ERCC1-independent manner.