Talin is a post-synaptic component of the rat neuromuscular junction.
Talin is a post-synaptic component of the rat neuromuscular junction.
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Talin 是大鼠神经肌肉接头的突触后成分。
DOI：
10.1016/0014-4827(86)90566-5
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发表时间：
1986
影响因子：
3.7
通讯作者：
Burridge,K
中科院分区：
文献类型：
--
作者：
Sealock,R;Paschal,B;Beckerle,M;Burridge,K
塔林蛋白是一种蛋白质，最近发现，在鸡砂囊，它发生在网站的肌动蛋白-质膜相互作用的几种细胞类型。在这些部位中的许多部位也会出现talin，可能与talin有关。在这项研究中，三种抗talin的抗血清被用来探测大鼠骨骼肌的神经肌肉接头，这也是黏着斑蛋白积累的一个网站。通过免疫荧光法，所有三种血清在大鼠膈肌的冷冻切片中强烈染色连接。在一些肌细胞中，连接外周边被轻度和不规则染色;其他人似乎没有在连接外染色。染色仍然在交界处，并增加在关节外区域的肌肉去神经6周前处死。在所有情况下的染色被取消的竞争与纯化的talin。通过免疫印迹法检测的一种血清在Mr 215 000（与鸡砂囊talin的值相同）处识别一种蛋白质，并且在Mr 190 000（对应于已知的talin蛋白水解片段）处识别第二种蛋白质的痕迹。我们的结论是，大鼠肌肉talin是类似的一般蛋白质结构，鸡砂囊talin，是一个突触后的神经肌肉接头的组成部分。
Talin is a protein, recently discovered in chicken gizzard, which occurs at sites of actin-plasma membrane interaction in several cell types. Vinculin also occurs at many of these sites, possibly in association with talin. In this study, three antisera against talin were used to probe the neuromuscular junction of rat skeletal muscle, which is also a site of vinculin accumulation. By immunofluorescence, all three sera stained the junction strongly in frozen sections of rat diaphragm. The extrajunctional periphery was lightly and irregularly stained in some muscle cells; others seemed not to be stained outside the junction. Staining remained at junctions and increased in extrajunctional regions of muscle denervated 6 weeks before sacrifice. The staining in all cases was abolished by competition with purified talin. One serum tested by immunoblotting recognized one protein atMr215 000 (identical with the value for chicken gizzard talin) and traces of a second atMr190 000 (corresponding to a known proteolytic fragment of talin). We conclude that rat muscle talin is similar in its general protein structure to chicken gizzard talin, and is a post-synaptic component of the neuromuscular junction.