Hepatocyte senescence predicts progression in non-alcohol-related fatty liver disease
Hepatocyte senescence predicts progression in non-alcohol-related fatty liver disease
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DOI：
10.1016/j.jhep.2012.10.031
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发表时间：
2013-03-01
影响因子：
25.7
通讯作者：
Alexander, Graeme
中科院分区：
文献类型：
--
作者：
Aravinthan, Aloysious;Scarpini, Cinzia;Alexander, Graeme
背景与目的：非酒精相关性脂肪性肝病（NAFLD）模型揭示了加速衰老的特征，如再生受损和肝细胞癌风险增加。加速衰老、疾病进展和临床结果之间的关系之前没有被调查过，这也是本次研究的主题。方法：采用定量荧光原位杂交和免疫组织化学方法，对70例NAFLD患者（105例）和60例对照者的肝脏切片进行端粒长度、核面积、DNA损伤和细胞周期期标志物的检测。结果：NAFLD组肝细胞端粒较对照组短(p
Background & Aims: Models of non-alcohol-related fatty liver disease (NAFLD) reveal features of accelerated ageing, such as impaired regeneration, and an increased risk of hepatocellular carcinoma. The relation between accelerated ageing, disease progression and clinical outcome has not been previously investigated and is the subject of the current study.Methods: Liver sections from 70 patients with NAFLD (105 biopsies) and 60 controls were studied for telomere length, nuclear area, DNA damage and cell cycle phase markers, using quantitative fluorescent in situ hybridization and immunohistochemistry.Results: Hepatocyte telomeres were shorter in NAFLD than controls (p