Tumor growth modulation by a monoclonal antibody to the epidermal growth factor receptor: immunologically mediated and effector cell-independent effects.
Tumor growth modulation by a monoclonal antibody to the epidermal growth factor receptor: immunologically mediated and effector cell-independent effects.
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发表时间：
1987-07
期刊：
影响因子：
11.2
通讯作者：
U. Rodeck;M. Herlyn;D. Herlyn;C. Molthoff;B. Atkinson;M. Varello;Z. Steplewski;H. Koprowski
中科院分区：
文献类型：
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作者：
U. Rodeck;M. Herlyn;D. Herlyn;C. Molthoff;B. Atkinson;M. Varello;Z. Steplewski;H. Koprowski
描述了一种IgG 2a同种型（425）的单克隆抗体，其与不同组织来源的人细胞上的表皮生长因子受体反应。单克隆抗体425在体外使用小鼠和人效应细胞介导肿瘤细胞毒性，并抑制表皮样癌（A 431）和结直肠癌（SW 948）衍生细胞系的体内肿瘤细胞生长。体外杀瘤作用与靶细胞上的抗原密度成正比。在高于1 nM的浓度下，单克隆抗体425抑制携带表皮生长因子受体的A 431细胞的生长，显示出对这些细胞的生长特性的表皮生长因子样激动剂活性。在生长抑制剂量的抗体或表皮生长因子的存在下生长的A431培养物显示S期细胞的相对数量明显减少。此外，用抗体处理的细胞在一些但不是所有测试的培养物中显示G2-M期细胞减少。
A monoclonal antibody of IgG2a isotype (425) is described that reacts with the epidermal growth factor receptor on human cells of different tissue origins. Monoclonal antibody 425 mediates tumor cytotoxicity in vitro using mouse and human effector cells and suppresses in vivo tumor cell growth of epidermoid (A 431) and colorectal (SW 948) carcinoma-derived cell lines. The tumoricidal effects in vitro are proportional to the antigen density on target cells. At concentrations higher than 1 nM, monoclonal antibody 425 inhibits growth of epidermal growth factor receptor-bearing A 431 cells, showing an epidermal growth factor-like agonist activity on the growth properties of these cells. A 431 cultures grown in the presence of growth-inhibiting doses of antibody or epidermal growth factor reveal a clear decrease of the relative number of cells in S phase. Additionally, cells treated with the antibody show a decrease of G2-M-phase cells in some, but not all, cultures tested.