IMMUNOPEROXIDASE LOCALIZATION OF GLIAL FIBRILLARY ACIDIC PROTEIN IN RADIAL GLIAL-CELLS AND ASTROCYTES OF THE DEVELOPING RHESUS-MONKEY BRAIN
IMMUNOPEROXIDASE LOCALIZATION OF GLIAL FIBRILLARY ACIDIC PROTEIN IN RADIAL GLIAL-CELLS AND ASTROCYTES OF THE DEVELOPING RHESUS-MONKEY BRAIN
复制标题
DOI：
10.1002/cne.901930316
复制
发表时间：
1980-01-01
影响因子：
2.5
通讯作者：
RAKIC, P
中科院分区：
文献类型：
--
作者：
LEVITT, P;RAKIC, P
采用过氧化物酶-抗过氧化物酶（PAP）免疫组化染色，利用胶质纤维酸性蛋白（GFA）特异性抗体，分析了恒河猴中枢神经系统的胶质发生[M]。从胚胎第38天（E38）到出生（E165）以及出生后第二个月不等。脑的所有主要分支都含有胶质细胞，通过黑褐色辣根过氧化物酶（HRP）反应产物的存在来识别。这种免疫细胞化学技术未对神经元成分进行染色。在发育过程中出现的第一类胶质细胞是放射状胶质细胞；放射状胶质纤维从它们的细胞体所在的脑室区和脑室下区呈扇形向外延伸至脑膜表面，在那里它们以圆锥形终足终止。这些胶质细胞在妊娠的前三分之一出现，在E41出现在脊髓和脑干中；间脑E45；在端脑和小脑中有E47。下一类出现的胶质细胞是小脑皮层的伯格曼胶质细胞，可以用E54染色。位于浦肯野细胞层下方的伯格曼胶质细胞产生平行的突起，并延伸至脑脊液表面。在大脑的每一个主要分支中，在胚胎发育过程中发生的主要构造-遗传变化期间，大量的细长胶质纤维不断改变其独特的模式，以保持恒定的脑室-脑脊膜表面关系。在nisl反染色切片中，观察到迁移神经元的柱与gfa阳性的径向和伯格曼胶质纤维并列。放射状胶质细胞在向成熟星形胶质细胞转变的过程中具有多种过渡形态。这种转变发生在特定胚胎年龄的每个结构中，并在神经元迁移开始消退后开始。神经发生完成后，星形胶质细胞的数量增加速度加快。在胚胎发育相对较早的阶段，径向胶质纤维的免疫组织化学定位表明，胶质细胞与神经元同时存在，这提高了至少2种不同的细胞前体细胞构成增殖区的可能性。在神经元迁移高峰期间，所有脑区都存在大量放射状胶质细胞，并且长形胶质纤维与迁移神经元之间存在密切的结构关系，这支持了胶质细胞在发育过程中对神经元元件的引导和分区化发挥重要作用的观点。
Peroxidase-antiperoxidase (PAP) immunohistochemical staining, using a specific antibody to the glial fibrillary acidic protein (GFA), was employed to analyze gliogenesis in the CNS of rhesus monkeys [M. mulatta] ranging in age from embryonic day 38 (E38) to birth (E165) and through the 2nd postnatal month. All major subdivisions of the brain contain glial cells, recognized by the presence of dark brown horseradish peroxidase (HRP) reaction product. Neuronal elements are not stained with this immunocytochemical technique. The 1st class of glial cells to appear during development are the radial glial cells; the radial glial fibers fan out from the ventricular and subventricular zones, where their cell bodies reside, to the pial surface where they terminate with conical endfeet. These glial cells appear within the 1st third of gestation, being present in the spinal cord and brain stem by E41; in the diencephalon by E45; and in the telencephalon and cerebellum by E47. The next class of glia to appear is the Bergmann glial cell of the cerebellar cortex, which can be stained by E54. Bergmann glial cells located below the Purkinje cell layer issue parallel processes which extend up to the pial surface. Within each major subdivision of the brain, massive numbers of elongated glial fibers continually alter their distinctive patterns to maintain constant ventricular-pial surface relationships during the major tecto-genetic changes which occur throughout embryonic development. In Nissl-counterstained sections columns of migrating neurons are observed juxtaposed to GFA-positive radial and Bergmann glial fibers. Radial glial cells assume a variety of transitional forms during the process of their transformation into mature astrocytes. This transformation occurs in each structure at specific embryonic ages and is initiated after neuronal migration has begun to subside. The number of astroglial cells increases at an accelerated pace after neurogenesis is complete. The immunohistochemical localization of radial glial fibers at relatively early stages of embryonic development indicates that glial cells are present concomitantly with neurons, raising the possibility that at least 2 distinct populations of cell precursors compose the proliferative zones. The demonstration of large numbers of radial glial cells in all brain regions during the peak of neuronal migration and a close structural realtionship between elongated glial fibers and migrating neurons support the concept that glia play a significant role in the guidance and compartmentalization of neuronal elements during development.