Phosphorus assay in column chromatography.
Phosphorus assay in column chromatography.
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DOI：
10.1016/s0021-9258(18)70226-3
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发表时间：
1959-03
期刊：
影响因子：
--
通讯作者：
G. R. Bartlett
中科院分区：
文献类型：
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作者：
G. R. Bartlett
在Fiske和Subbarow的方法的这种修改中，高达2 ml。的样品和0.5ml。10 NH 2SO 4在150°至160°C下加热。加入两滴30%的H2 O2并将溶液再加热1 1/2小时。0.22%的氯化铵和0.2 ml.的试剂（0.5g. 1-氨基-2-萘酚-4-磺酸与200 ml. NaHSO 3和1g.将溶液在沸水中加热7 min，并在830 mµ处测量光密度。并给出了无机磷酸盐的测定方法。当它与磷酸酯混合时，最初的Fiske Subbarow方法是最可靠的，但并不理想。A.赫普本。
In this modification of the method of Fiske and Subbarow, up to 2 ml. of the sample and 0.5 ml. 10NH2SO4were heated at 150°to 160°C. for at least 3 hr. Two drops of 30 per cent. H2O2were added and the solution was heated for another 1 1/2 hr. After addition of 4.6 ml. 0.22 per cent. ammonium molybdate and 0.2 ml. of the reagent (0.5 g. 1-amino-2-naphthol-4-sulphonic acid mixed with 200 ml. 15 per cent. NaHSO3and 1 g. Na2SO3and the solution filtered) the solution was heated for 7 min. in boiling water and the optical density was measured at 830 mµ. A procedure for inorganic phosphate is given also. When it was mixed with phosphate esters the original Fiske Subbarow method was most reliable but not ideal. A. Hepburn.