THE ACTION OF 9 CHELATORS ON IRON-DEPENDENT RADICAL DAMAGE
THE ACTION OF 9 CHELATORS ON IRON-DEPENDENT RADICAL DAMAGE
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DOI：
10.3109/10715769409147506
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发表时间：
1994-01-01
影响因子：
3.3
通讯作者：
NICHOLSON, P
中科院分区：
文献类型：
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作者：
DEAN, RT;NICHOLSON, P
在五个系统中测试了九种铁螯合剂对自由基生成和转化的影响（螯合剂：铁摩尔比为 0.1 至 10）。刺激作用可能会区分有毒螯合剂和更安全的螯合剂。研究了代表铁（亚铁和三价铁）可用性不同方面的自由基生成反应：a）与过氧化氢反应使苯甲酸羟基化； b) 抗坏血酸的氧化； c) 与过氧化氢反应以裂解蛋白质； d) 与过氧化氢反应以允许放大的化学发光； e) 诱导线粒体膜脂质过氧化。使用的化合物为 HBED、CP130、Desferal、EDTA、吡啶腙 (CGP 43'902B)、Ferrozine、CP 94 (CGP 46'700)、L1 (CGP 37 391) 和红酵母酸 (CGP 45 274)。只有六齿化合物 HBED、CP130 和 Desferal 具有一致的抑制性（“保护性”）。保护性化合物在自由基通量过程中也明显比其他螯合剂更稳定。
Nine iron chelators were tested in five systems for their effects on radical-generation and conversion at chelator: iron molar ratios from 0.1 to 10. Stimulatory actions might distinguish toxic from safer chelators. Radical-generating reactions which represent different aspects of iron (ferrous and ferric) availability were studied: a) the reaction with hydrogen peroxide to hydroxylate benzoate; b) the oxidation of ascorbate; c) the reaction with hydrogen peroxide to fragment proteins; d) the reaction with hydrogen peroxide to permit amplified chemiluminescence; and e) the induction of peroxidation of mitochondrial membrane lipids. The compounds used were HBED, CP130, Desferal, EDTA, pyridine-hydrazone (CGP 43'902B), Ferrozine, CP 94 (CGP 46'700), L1 (CGP 37 391) and rhodotorulic acid (CGP 45 274). Only the hexadentate compounds HBED, CP130 and Desferal were uniformly inhibitory (''protective''). The protective compounds were also apparently more stable during radical fluxes than the other chelators.