Isolation and characterization of human malignant glioma cells from histologically normal brain
Isolation and characterization of human malignant glioma cells from histologically normal brain
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DOI：
10.3171/jns.1997.86.3.0525
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发表时间：
1997-03-01
影响因子：
4.1
通讯作者：
Chicoine, MR
中科院分区：
文献类型：
--
作者：
Silbergeld, DL;Chicoine, MR
脑侵袭阻止了恶性胶质瘤的完全手术切除；然而，来自遥远的、组织学正常的脑侵袭细胞以前没有被分离、培养和鉴定。为了评估侵袭性的人类恶性胶质瘤细胞，作者建立了肉眼肿瘤和组织学正常脑组织的培养。三名男性和一名女性，平均年龄67岁，接受了两次额叶和两次颞叶肿瘤切除术，得到了大体肿瘤和组织学正常的脑组织标本。每个标本均取自肉眼肿瘤至少4厘米处。标本被分割：一部分被送去进行神经病理学评估(3例多形性胶质母细胞瘤和1例少突胶质细胞瘤)，一部分被用来建立细胞系。在四个细胞培养对中，有三个大体肿瘤标本和组织学正常脑组织标本在形态上是相同的。组织化学染色特征在每一对内都是一致的，并且与送去进行神经病理学评估的标本相比都是一致的。培养显示软琼脂糖和肿瘤核型中的非锚定生长。在四组培养对中的三组中，组织学正常脑组织的培养生长率高于肉眼肿瘤的生长率。虽然观察到的组织学正常脑培养细胞生长率的增加确实与体内行为相关，但这些发现证实了先前报道的侵袭性胶质瘤细胞的干细胞潜力。用放射碟法，在大体肿瘤培养物和组织学正常脑组织之间没有发现显著差异。总之，肿瘤细胞是从距离大体肿瘤4 cm以上的组织学正常脑组织中培养出来的，这表明对浸润性胶质瘤细胞的标准组织病理学鉴定相对不敏感(因此冰冻切片对切除边缘的评估是不够的)。来自大体肿瘤和组织学正常脑组织的细胞系通常在组织学上是相同的，表现出相同的运动性，但具有不同的生长速度。
Brain invasion prevents complete surgical extirpation of malignant gliomas; however, invasive cells from distant, histologically normal brain previously have not been isolated, cultured, and characterized. To evaluate invasive human malignant glioma cells, the authors established cultures from gross tumor and histologically normal brain. Three men and one woman, with a mean age of 67 years, underwent two frontal and two temporal lobectomies for tumors, which yielded specimens of both gross tumor and histologically normal brain. Each specimen was acquired a minimum of 4 cm from the gross tumor. The specimens were split: a portion was sent for neuropathological evaluation (three glioblastomas multiforme and one oligodendroglioma) and a portion was used to establish cell lines. Morphologically, the specimens of gross tumor and histologically normal brain were identical in three of the four cell culture pairs. Histochemical staining characteristics were consistent both within each pair and when compared with the specimens sent for neuropathological evaluation. Cultures demonstrated anchorage-independent growth in soft agarose and neoplastic karyotypes. Growth rates in culture were greater for histologically normal brain than for gross tumor in three of the four culture pairs. Although the observed increases in growth rates of histologically normal brain cultures do pot correlate with in vivo behavior, these findings corroborate the previously reported stem cell potential of invasive glioma cells. Using the radial dish assay, no significant differences in motility between cultures of gross tumor and histologically normal brain were found.In summary, tumor cells were cultured from histologically normal brain acquired from a distance greater than 4 cm from the gross tumor, indicating the relative insensitivity of standard histopathological identification of invasive glioma cells (and hence the inadequacy of frozen-section evaluation of resection margins). Cell lines derived from gross tumor and histologically normal brain were usually histologically identical and demonstrated equivalent motility, but had different growth rates.