Diagnostic Usefulness of p16/CDKN2A FISH in Distinguishing Between Sarcomatoid Mesothelioma and Fibrous Pleuritis
Diagnostic Usefulness of p16/CDKN2A FISH in Distinguishing Between Sarcomatoid Mesothelioma and Fibrous Pleuritis
复制标题
DOI：
10.1309/ajcpt94jvwihbkrd
复制
发表时间：
2013-01-01
影响因子：
3.5
通讯作者：
Tagawa, Masatoshi
中科院分区：
文献类型：
--
作者：
Wu, Di;Hiroshima, Kenzo;Tagawa, Masatoshi
根据组织学区分肉瘤样间皮瘤和纤维性胸膜炎很困难，特别是当通过活检检查的肿瘤组织量较小且免疫组织化学检查不确定时。我们研究了荧光原位杂交 (FISH) 删除 p16 和聚合酶链反应 p16 高甲基化对于恶性胸膜间皮瘤 (MPM) 的诊断和预后的有用性。我们分析了 50 例 MPM，包括 22 例肉瘤样间皮瘤病例和 10 例纤维性胸膜炎病例。基于使用纤维性胸膜炎样本的 FISH 信号传导模式，我们将纯合缺失模式的截止值设置为 14.4%。 55.6% 的上皮样间皮瘤 (10/18) 和所有肉瘤样间皮瘤 (22/22) 中纯合缺失模式的百分比高于 14.4%。在 34 个提供信息的病例中，有 7 个（20.6%）观察到 p16 甲基化。 p16 FISH 分析可以是区分肉瘤样间皮瘤和纤维性胸膜炎的可靠测试，也是 MPM 的预后因素。
The distinction between sarcomatoid mesothelioma and fibrous pleuritis is difficult based on histology, especially when the amount of tumor tissue examined via biopsy is small and immunohistochemical examination is inconclusive. We studied the usefulness of deletion of p16 with fluorescence in situ hybridization (FISH) and p16 hypermethylation with polymerase chain reaction for the diagnosis and prognosis of malignant pleural mesothelioma (MPM). We analyzed 50 MPMs, including 22 sarcomatoid mesothelioma cases and 10 fibrous pleuritis cases. We set the cutoff value of homozygous deletion pattern as 14.4% based on FISH signaling patterns using samples of fibrous pleuritis. The percentage of homozygous deletion pattern was higher than 14.4% in 55.6% of the epithelioid mesotheliomas (10/18) and in all of the sarcomatoid mesotheliomas (22/22). Methylation of p16 was observed in 7 (20.6%) of 34 informative cases. p16 FISH analysis can be a reliable test for distinguishing between sarcomatoid mesothelioma and fibrous pleuritis and a prognostic factor for MPM.