Columnar DNA Superlattices in Lamellar o-Ethylphosphatidylcholine Lipoplexes: Mechanism of the Gel-Liquid Crystalline Lipid Phase Transition
Columnar DNA Superlattices in Lamellar o-Ethylphosphatidylcholine Lipoplexes: Mechanism of the Gel-Liquid Crystalline Lipid Phase Transition
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DOI：
10.1021/nl049191k
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发表时间：
2004-07
期刊：
影响因子：
10.8
通讯作者：
R. Koynova;R. Macdonald
中科院分区：
文献类型：
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作者：
R. Koynova;R. Macdonald
DNA在阳离子O-乙基磷脂酰胆碱的脂复合物的低温凝胶相中排列成相互交叉的脂质双层之间的矩形柱状超晶格。在液晶脂质相中没有观察到DNA有序化的层间相关性。脂质的凝胶-液晶相变诱导DNA近晶阵列的收缩，该收缩与由交错-非交错过渡引起的膜表面的正电荷密度增加相匹配。过渡过程通过一个中间状态进行，该中间状态具有扩展的层状重复周期。
DNA arranges into rectangular columnar superlattices between interdigitated lipid bilayers in the low-temperature gel phase of the lipoplexes of the cationic o-ethylphosphatidylcholines. The interlamellar correlation in the DNA ordering is not observed within the liquid crystalline lipid phase. The gel-liquid crystalline phase transition of the lipid induces a contraction of the DNA smectic array that matches the increased positive charge density of the membrane surface caused by the interdigitated-noninterdigitated transition. The transition proceeds via an intermediate state that has an expanded lamellar repeat period.