Human Gut Bacterial Communities Are Altered by Addition of Cruciferous Vegetables to a Controlled Fruit- and Vegetable-Free Diet
Human Gut Bacterial Communities Are Altered by Addition of Cruciferous Vegetables to a Controlled Fruit- and Vegetable-Free Diet
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DOI：
10.3945/jn.109.108191
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发表时间：
2009-09-01
影响因子：
4.2
通讯作者：
Lampe, Johanna W.
中科院分区：
文献类型：
--
作者：
Li, Fei;Hullar, Meredith A. J.;Lampe, Johanna W.
在人类肠道中，肠道细菌代谢通常作为能量来源的食物成分。这些成分有可能影响肠道细菌群落组成。十字花科蔬菜，如花椰菜和卷心菜，含有独特的化合物，可以被肠道细菌利用。例如，芥子油苷可以被某些细菌水解，膳食纤维可以被一系列物种发酵。我们假设，十字花科蔬菜消费将改变某些细菌的生长，从而改变细菌群落组成。我们在一项随机、交叉、控制喂养的研究中检验了这一假设。粪便样本收集了17名参与者在2 14天的摄入期结束时：低植物化学物质，低纤维的基础饮食（即精制谷物没有水果或蔬菜）和高（“双”）十字花科蔬菜饮食[基础饮食+ 14克十字花科蔬菜/（公斤体重.d）]。采用细菌16 S核糖体RNA基因末端限制性片段长度多态性（tRFLP）方法和核苷酸测序分析粪便细菌组成。使用阻断多应答排列程序分析，我们发现两个消费期之间的总体细菌群落组成不同（delta = 0.603; P = 0.011）。非度量多维尺度排序分析表明，细菌群落对十字花科蔬菜的反应是个体特异性的。使用指示物种分析鉴定了表征每种饮食的特定tRFLP片段。通过基因测序将对应于这些片段的推定物种鉴定为霍氏真杆菌（Eubacterium hallii）、屎乳杆菌（Phascolarctobacterium faecium）、伯克霍尔德菌属（Burkholderiales spp.）Alistipes putredinis和Eggerthella spp.总之，人类肠道细菌群落组成改变了十字花科蔬菜消费，这可能最终影响肠道代谢的生物活性食品成分和主机暴露于这些化合物。J.营养139：1685-1691，2009.
In the human gut, commensal bacteria metabolize food components that typically serve as energy sources. These components have the potential to influence gut bacterial community composition. Cruciferous vegetables, such as broccoli and cabbage, contain distinctive compounds that can be utilized by gut bacteria. For example, glucosinolates can be hydrolyzed by certain bacteria, and dietary fibers can be fermented by a range of species. We hypothesized that cruciferous vegetable consumption would alter growth of certain bacteria, thereby altering bacterial community composition. We tested this hypothesis in a randomized, crossover, controlled feeding study. Fecal samples were collected from 17 participants at the end of 2 14-d intake periods: a low-phytochemical, low-fiber basal diet (i.e. refined grains without fruits or vegetables) and a high ("double") cruciferous vegetable diet [basal diet + 14 g cruciferous vegetables/(kg body weight.d)]. Fecal bacterial composition was analyzed by the terminal restriction fragment length polymorphism (tRFLP) method using the bacterial 16S ribosomal RNA gene and nucleotide sequencing. Using blocked multi-response permutation procedures analysis, we found that overall bacterial community composition differed between the 2 consumption periods (delta = 0.603; P = 0.011). The bacterial community response to cruciferous vegetables was individual-specific, as revealed by nonmetric multidimensional scaling ordination analysis. Specific tRFLP fragments that characterized each of the diets were identified using indicator species analysis. Putative species corresponding to these fragments were identified through gene sequencing as Eubacterium hallii, Phascolarctobacterium faecium, Burkholderiales spp., Alistipes putredinis, and Eggerthella spp. In conclusion, human gut bacterial community composition was altered by cruciferous vegetable consumption, which could ultimately influence gut metabolism of bioactive food components and host exposure to these compounds. J. Nutr. 139: 1685-1691, 2009.