Floppy SOX: mutual induced fit in hmg (high-mobility group) box-DNA recognition.
Floppy SOX: mutual induced fit in hmg (high-mobility group) box-DNA recognition.
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DOI：
10.1210/mend.15.3.0617
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发表时间：
2001-03
影响因子：
--
通讯作者：
Michael A. Weiss
中科院分区：
文献类型：
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作者：
Michael A. Weiss
高迁移率族（HMG）盒定义了与人类发育和疾病有关的广泛兴趣的DNA弯曲基序。L形结构的主翼和次翼为DNA弯曲提供了模板。如在TATA结合蛋白和多种因子家族中，在碱基对之间插入一个或多个侧链诱导DNA扭结。HMG盒结合在DNA小沟中，并且可能对DNA序列或扭曲的DNA结构具有特异性。而非序列特异性结构域的角结构是有序的，游离SRY和相关的常染色体SOX结构域是部分无序的。观察表明，小翼缺乏一个固定的三级结构激发的假设，DNA弯曲和蛋白质结构的稳定定义一个耦合的过程。我们进一步提出，SOX-DNA识别中的相互诱导适合是DNA弯曲的序列依赖性的基础，并且能够诱导启动子特异性结构。
The high-mobility group (HMG) box defines a DNA-bending motif of broad interest in relation to human development and disease. Major and minor wings of an L-shaped structure provide a template for DNA bending. As in the TATA-binding protein and a diverse family of factors, insertion of one or more side chains between base pairs induces a DNA kink. The HMG box binds in the DNA minor groove and may be specific for DNA sequence or distorted DNA architecture. Whereas the angular structures of non-sequence-specific domains are well ordered, free SRY and related autosomal SOX domains are in part disordered. Observations suggesting that the minor wing lacks a fixed tertiary structure motivate the hypothesis that DNA bending and stabilization of protein structure define a coupled process. We further propose that mutual induced fit in SOX-DNA recognition underlies the sequence dependence of DNA bending and enables the induction of promoter-specific architectures.