Methionine and choline regulate the metabolic phenotype of a ketogenic diet
Methionine and choline regulate the metabolic phenotype of a ketogenic diet
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DOI：
10.1016/j.molmet.2013.07.003
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发表时间：
2013-08-01
影响因子：
8.1
通讯作者：
Maratos-Flier, Eleftheria
中科院分区：
文献类型：
--
作者：
Pissios, Pavlos;Hong, Shangyu;Maratos-Flier, Eleftheria
低碳水化合物生酮饮食通常被用作低脂饮食的减肥替代品，但对这些饮食的生理和分子适应性尚不完全清楚。据推测，生酮饮食（KD）的代谢表型是由碳水化合物的缺乏和高脂肪含量引起的，然而在啮齿动物中，KD的蛋白质含量影响体重增加和酮症。在这项研究中，我们研究了蛋氨酸和胆碱在介导KD的代谢效应中的作用。我们已经发现，胆碱是更有效地减少肝脂肪变性的KD喂养小鼠比蛋氨酸。另一方面，补充蛋氨酸比胆碱更有效地恢复体重增加，并使KD喂养小鼠肝脏中几种脂肪酸和炎症基因的表达正常化。我们的研究结果表明，胆碱和蛋氨酸限制，而不是碳水化合物限制的基础上KD的许多代谢作用。(C)2013作者由爱思唯尔有限公司出版。CCBY-NC-SA许可下的开放获取
Low-carbohydrate ketogenic diets are commonly used as weight loss alternatives to low-fat diets, however the physiological and molecular adaptations to these diets are not completely understood. It is assumed that the metabolic phenotype of the ketogenic diet (KD) is caused by the absence of carbohydrate and high fat content, however in rodents the protein content of KD affects weight gain and ketosis. In this study we examined the role of methionine and choline in mediating the metabolic effects of KD. We have found that choline was more effective than methionine in decreasing the liver steatosis of KD-fed mice. On the other hand, methionine supplementation was more effective than choline in restoring weight gain and normalizing the expression of several fatty acid and inflammatory genes in the liver of KD-fed mice. Our results indicate that choline and methionine restriction rather than carbohydrate restriction underlies many of the metabolic effects of KD. (C) 2013 The Authors. Published by Elsevier GmbH. Open access under CCBY-NC-SA license