Protective immunization against visceral leishmaniasis using Leishmania sterol 24-c-methyltransferase formulated in adjuvant
Protective immunization against visceral leishmaniasis using Leishmania sterol 24-c-methyltransferase formulated in adjuvant
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DOI：
10.1016/j.vaccine.2007.08.001
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发表时间：
2007-10-16
期刊：
影响因子：
5.5
通讯作者：
Reed, Steven G.
中科院分区：
文献类型：
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作者：
Goto, Yasuyuki;Bogatzki, Lisa Y.;Reed, Steven G.
我们在此介绍利什曼原虫甾醇 24-c-n-i 乙基转移酶 (SMT) 的鉴定和表征，以及用 MPL (R)-SE 配制的这种 Ag 免疫小鼠的保护数据。血清学评估显示 VL 患者可识别 SMT。使用该候选疫苗加 MPL (R)-SE 免疫的 C57BL/6 小鼠表现出 Ag 特异性 Th1 免疫反应，其特征是在体外再次暴露于特异性 Ag 后，会大量产生 IFN-γ。在用婴儿乳杆菌攻击后，与未免疫小鼠或单独注射佐剂的小鼠相比，用 SMT 加 MPL (R)-SE 免疫的小鼠的脾脏和肝脏中的寄生虫负荷显着降低。结果表明，SMT/MPL (R)-SE 可以成为对抗 VL 的有效候选疫苗。 (c) 2007 Elsevier Ltd. 保留所有权利。
We present here the identification and characterization of Leishmania sterol 24-c-n-iethyltransferase (SMT), as well as data on protection of mice immunized with this Ag formulated in MPL (R)-SE. Serological evaluation revealed that SMT is recognized by VL patients. C57BL/6 mice immunized with this vaccine candidate plus MPL (R)-SE showed Ag-specific Th1 immune responses characterized by robust production of IFN-gamma upon specific Ag re-exposure in vitro. Upon challenge with L. infantum, mice immunized with SMT plus MPL (R)-SE showed significant lower parasite burdens in both spleens and livers compared with non-immunized mice or mice injected with adjuvant alone. The results indicate that SMT/MPL (R)-SE can be an effective vaccine candidate for use against VL. (c) 2007 Elsevier Ltd. All rights reserved.