Effect of mast cell degranulation on chicken ileal ion transport in vitro
Effect of mast cell degranulation on chicken ileal ion transport in vitro
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DOI：
10.1093/ps/86.5.843
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发表时间：
2007-05-01
期刊：
影响因子：
4.4
通讯作者：
Campion, D. P.
中科院分区：
文献类型：
--
作者：
Collins, C. B.;McGrath, J.;Campion, D. P.
组胺是哺乳动物炎症反应的主要介质。肠道肥大细胞脱颗粒导致肥大细胞介质如组胺的释放。组胺通过特定的组胺受体刺激多种哺乳动物组织上皮细胞的离子转运。本研究的目的是评估组织肥大细胞和外源性组胺在调节禽类粘膜离子转运中的潜在作用。采用酶联免疫吸附试验和Ussing小室技术测定肉鸡回肠组织胺释放和肥大细胞脱颗粒的分泌反应。使用化合物48/80(15微克/毫升)对粘膜肥大细胞进行药理脱颗粒可导致组胺释放和跨壁短路电流的即刻增加，化合物48/80的反应受到快速反应的影响，在组胺H拮抗剂美吡拉明的存在下显著降低，但不受环氧合酶抑制剂吡罗昔康的影响。预先与肥大细胞稳定剂酮替芬孵育可阻止化合物48/80引起的跨壁短路电流增加。综上所述，禽类肠道肥大细胞脱颗粒可能导致组胺释放，通过组胺H受体激活刺激上皮细胞离子转运。虽然前列腺素E-2在禽类小肠上皮细胞中是一种强有力的促分泌剂，但前列腺素类物质的产生在肥大细胞介导的上皮离子转运中似乎扮演着很小的角色。
Histamine is a primary mediator of the inflammatory response in mammals. Degranulation of intestinal mast cells results in the release of mast cell mediators such as histamine. Histamine stimulates epithelial ion transport in a range of mammalian tissues via specific histamine receptors. The aim of this study was to assess a potential role of tissue mast cells and of exogenous histamine in the regulation of ion transport in avian mucosa. Broiler chicken ileal histamine release and secretory responses to mast cell degranulation were determined in vitro with the use of ELISA and Ussing chamber techniques. Pharmacological degranulation of mucosal mast cells using compound 48/80 (15 mu g/mL) resulted in histamine release and an immediate-onset transient increase in transmural short-circuit current, The response to compound 48/80 was subject to tachyphylaxis and was significantly reduced in the presence of the histamine H, antagonist mepyramine, but was unaffected by the cyclooxygenase inhibitor piroxicam. Prior incubation with the mast cell stabilizer ketotifen prevented compound 48/80-induced increase in transmural short-circuit current. In conclusion, degranulation of avian intestinal mast cells would appear to result in histamine release that stimulates epithelial ion transport via histamine H, receptor activation. Although prostaglandin E-2 is a potent secretagogue in the avian small intestine epithelium, prostanoid production appears to have little role to play in mast cell-mediated epithelial ion transport.