STRUCTURAL CHARACTERIZATION OF THE N-LINKED CARBOHYDRATE CHAINS FROM MOUSE ZONA-PELLUCIDA GLYCOPROTEINS ZP2 AND ZP3
STRUCTURAL CHARACTERIZATION OF THE N-LINKED CARBOHYDRATE CHAINS FROM MOUSE ZONA-PELLUCIDA GLYCOPROTEINS ZP2 AND ZP3
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DOI：
10.1016/0304-4165(93)90018-4
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发表时间：
1993-11-28
期刊：
影响因子：
--
通讯作者：
NAKANO, M
中科院分区：
文献类型：
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作者：
NOGUCHI, S;NAKANO, M
N-连接的小鼠透明质酸糖蛋白ZP 2和ZP 3的寡糖被制备为吡啶胺化衍生物。阴离子交换HPLC显示，95%或更多的是酸性的。约80%的这些酸性链被中和与节杆菌尿素唾液酸酶消化和少量的硫酸根被发现在残留的酸性链。通过尺寸分级HPLC将来自ZP 2和ZP 3的中和级分（SN级分）类似地分级分离成在非还原区域中具有不同数量LacNAc的许多峰。来自ZP 2的SN级分被内切-β-半乳糖苷酶切割成核心和非还原区。三和四触角复杂型链与Fuc残基占主导地位的核心区域。另一方面，从非还原区获得了四种含有LacNAc序列的片段。唾液酸显示与核心片段以及非还原片段连接。ZP 3的N-连接寡糖被认为具有与ZP 2的那些基本上相同的结构。
N-linked oligosaccharides of mouse zona pellucida glycoproteins ZP2 and ZP3 were prepared as pyridylaminated derivatives. Anion-exchange HPLC revealed that 95% or more of them are acidic. About 80% of these acidic chains were neutralized by digestion with Arthrobacter ureafaciens sialidase and a small amount of sulfate was found in the residual acidic chains. The neutralized fractions (SN fractions) from ZP2 and ZP3 were similarly fractionated by size-fractionation HPLC into many peaks having different numbers of LacNAc in the non-reducing regions. The SN fractions from ZP2 were cleaved by endo-beta-galactosidase into the core and non-reducing regions. The tri- and tetra-antennary complex-type chains with a Fuc residue were predominant in the core region. On the other hand, four kinds of fragment containing the LacNAc sequence were obtained from the non-reducing region. Sialic acids were shown to be linked to the core fragments, as well as the non-reducing fragments. The N-linked oligosaccharides of ZP3 were suggested to have essentially the same structures as those of ZP2.