Opioid induced non-photic phase shifts of hamster circadian activity rhythms
Opioid induced non-photic phase shifts of hamster circadian activity rhythms
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DOI：
10.1016/s0006-8993(00)02304-0
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发表时间：
2000-08-11
期刊：
影响因子：
2.9
通讯作者：
Gannon, RL
中科院分区：
文献类型：
--
作者：
Byku, M;Gannon, RL
仓鼠的昼夜节律起搏器的相位可以通过在主观一天的晚些时候应用某些非光刺激来改变。在视交叉上核中，从丘脑的膝间起搏器到昼夜节律起搏器的投射被认为介导了某些类型的非光性相移刺激。在仓鼠中，这种投射对神经肽Y和脑啡肽都是免疫反应的。其他实验室之前的工作表明，神经肽Y的应用能够在不应用光的情况下改变昼夜节律的相移。本研究旨在确定脑啡肽能化合物是否同样具有非光相移仓鼠活动节律的能力。仓鼠在恒定的黑暗条件下饲养，记录轮子的昼夜运行活动。在昼夜节律8和10，对kappa、u和Delta阿片受体具有选择性的激动剂和拮抗剂系统地应用于仓鼠，以确定它们是否能够引起轮子运行活动节律的相移。在所测试的化合物中，只有三角洲阿片激动剂BW373U86显著影响昼夜节律。BW373U86期仓鼠跑轮活动节律提前约45分钟，尽管药物应用后的总活动水平没有受到显著影响。尽管昼夜节律没有改变，但在给予某些u和kappa类阿片后，轮子运行活动量发生了变化。这些结果表明，脑啡肽类似的Delta阿片激动剂能够在金黄地鼠的活动节律中产生非光相移，并且阿片类药物可以独立地影响金黄地鼠的昼夜节律和活动水平。(C)2000 Elsevier Science B.V.保留所有权利。
The phase of the circadian pacemaker in hamsters can be shifted by the application of certain non-photic stimuli late in the subjective day. A projection from the intergeniculate leafier of the thalamus to the circadian pacemaker in the suprachiasmatic nucleus is believed to mediate some types of non-photic phase-shifting stimuli. In hamsters, this projection is immunoreactive to both Neuropeptide Y and enkephalin. Previous work in other laboratories has shown that Neuropeptide Y administration is capable of phase shifting circadian rhythms without the application of light. The present study was undertaken to determine if enkephalinergic compounds likewise have the ability to non-photically phase shift hamster activity rhythms. Hamsters were maintained under conditions of constant darkness and circadian wheel running activity was recorded. Agonists and antagonists selective for kappa, mu, and delta opioid receptors were systemically applied without light to hamsters at circadian times 8 and 10 to determine if they were able to elicit phase shifts in wheel running activity rhythms. Of the compounds tested, only the delta opioid agonist BW373U86 significantly affected circadian phase. BW373U86 phase advanced hamster wheel running activity rhythms by approximately 45 min, although total activity levels following drug application were not significantly affected. Changes in the amount of wheel running activity were detected after administration of some mu and kappa opioids, although the circadian phase was not altered. These results indicate that enkephalin-mimetic delta opioid agonists are capable of producing non-photic phase shifts in hamster activity rhythms, and that opioids can independently affect circadian phase and activity levels in hamsters. (C) 2000 Elsevier Science B.V. All rights reserved.