Intraperitoneal anaesthesia with propofol, medetomidine and fentanyl in mice
Intraperitoneal anaesthesia with propofol, medetomidine and fentanyl in mice
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DOI：
10.1258/la.2008.007036
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发表时间：
2009-01-01
影响因子：
2.4
通讯作者：
Antunes, L. M.
中科院分区：
文献类型：
--
作者：
Alves, H. C.;Valentim, A. M.;Antunes, L. M.
在寻找用于小鼠的安全麻醉方案时，快速恢复至关重要。异丙酚是一种短效麻醉剂，可使患者平稳、快速地恢复。最近在我们实验室进行的一项研究表明，腹膜内（i. p.）丙泊酚与速效阿片样物质联合给药不能提供足够稳定的麻醉。在本实验中，我们假设额外应用美托咪定会增加肌肉松弛和镇痛。将54只雄性CD 1小鼠分为6组（每组5只）和3组（每组8只），用于测试丙泊酚、美托咪定和芬太尼的9种不同组合，这些组合在诱导后30 min经腹腔注射给药，并用阿替美唑逆转。这些复方制剂按以下方式组成：丙泊酚75 mg/kg、美托咪定1和2 mg/kg以及芬太尼0.1、0.15和0.2 mg/kg。沿着记录麻醉深度、翻正反射丧失、足退缩反射丧失、脉搏率和呼吸率以及恢复时间和质量。异丙酚和美托咪定的组合提供了可预测的诱导、催眠和肌肉松弛，但手术麻醉。(loss足回缩反射）。芬太尼的加入增加了镇痛，导致手术麻醉。我们的结论是，75/1/0.2 mg/kg的丙泊酚、美托咪定和芬太尼的组合分别是用于小鼠腹腔内麻醉的安全、简单和可逆的技术，提供了15 min的手术窗口和30 min的束缚，恢复快。
Fast recoveries are essential when looking for a safe anaesthetic protocol to use on mice. Propofol is a short-acting anaesthetic agent, which provides a smooth, fast recovery. A recent study carried out in our laboratory showed that the intraperitoneal (i.p.) administration of propofol combined with a fast-acting opioid does not provide a sufficiently stable anaesthesia. In this experiment, we hypothesized that the additional application of medetomidine would increase muscle relaxation and analgesia. Fifty-four male CD1 mice, divided into six groups of five and three groups of eight, were used to test nine different combinations of propofol, medetomidine and fentanyl administered i.p. and reversed with atipamezole 30 min after induction. These combinations were composed in the following manner: propofol 75 mg/kg, medetomidine 1 and 2 mg/kg and fentanyl 0.1, 0.15 and 0.2 mg/kg. The depth of anaesthesia, loss of righting reflex, loss of pedal withdrawal reflex, pulse rate and respiratory rate were recorded along with the duration and quality of the recovery. The combination of propofol and medetomidine provided a predictable induction, hypnosis and muscle relaxation, but surgical anaesthesia. (loss of pedal withdrawal reflex) was not achieved. The addition of fentanyl increased analgesia leading to surgical anaesthesia. We concluded that a combination of 75/1/0.2 mg/kg of propofol, medetomidine and fentanyl, respectively, is a safe, easy and reversible technique for i.p. anaesthesia in mice, providing a surgical window of 15 min and restraint for 30 min with a fast recovery.