Temperature and ion dual responsive biphenyl-dipeptide supramolecular hydrogels as extracellular matrix mimic-scaffolds for cell culture applications
Temperature and ion dual responsive biphenyl-dipeptide supramolecular hydrogels as extracellular matrix mimic-scaffolds for cell culture applications
复制标题
温度和离子双响应联苯二肽超分子水凝胶作为细胞外基质模拟支架用于细胞培养应用
DOI：
10.1039/c7tb00576h
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发表时间：
2017-05-28
影响因子：
7
通讯作者：
Zhang, Xingdong
中科院分区：
文献类型：
--
作者：
Bian, Shaoquan;Cai, Hanxu;Zhang, Xingdong
由芳香族短肽胶凝剂组成的刺激响应型超分子水凝胶因其稳定的化学结构、简单便捷的合成路线以及对外界刺激的智能响应而受到生物医学领域的广泛关注。在本文中，几种二肽与联苯乙酸（BPAA）偶联，通过标准固相肽合成生成芳香族短肽化合物。这些BPAA-二肽化合物与常用的Fmoc-二肽和Nap-二肽化合物表现出明显不同的凝胶行为，但只有BPAA-二苯丙氨酸能够通过温度转换或离子诱导形成均匀透明的水凝胶。利用联苯基不仅扩大了芳香族分子作为芳香族短肽胶凝剂构建块的范围，而且证明了芳香族分子在自组装过程中的关键作用。此外，通过加热-冷却或加盐引发的超分子水凝胶可用作细胞外基质（ECM）模拟支架，以支持生理条件下2D/3D培养中L929细胞的粘附生长和增殖，展示了其在再生医学中的潜在应用。
Stimuli-responsive supramolecular hydrogels composed of aromatic short peptide gelators have attracted intensive attention in the field of biomedicine because of their stable chemical structure, simple and convenient synthetic route and intelligent response to external stimuli. In this paper, several dipeptides were coupled to biphenylacetic acid (BPAA) to generate aromatic short peptide compounds through the standard solid phase peptide synthesis. These BPAA-dipeptide compounds presented clearly different gelation behaviors from the generally employed Fmoc-dipeptide and Nap-dipeptide compounds, but only BPAA-diphenylalanine was able to form homogeneous and transparent hydrogels through temperature switching or ion induction. Utilizing the biphenyl group not only expanded the scope of aromatic molecules serving as building blocks of aromatic short peptide gelators but also demonstrated the critical role of aromatic molecules in the self-assembling process. Moreover, supramolecular hydrogels initiated by heating-cooling or salt addition could be exploited as extracellular matrix (ECM) mimic scaffolds to support the adhesive growth and proliferation of L929 cells in 2D/3D culture under physiological conditions, demonstrating their potential applications in regenerative medicine.